PMID: 6166615May 1, 1981Paper

Study of liver differentiation in vitro

The Journal of Cell Biology
J Y Chou, S E Schlegel-Haueter

Abstract

A clonal rat fetal liver cell line that expresses the functions of differentiated liver cells under controllable conditions has been established. Normal fetal liver cells were transformed by a temperature-sensitive A (tsA) mutant (tsA209) of simian virus 40. At the permissive temperature (33 degrees C), the tsA209-transformed liver cell line (RLA209-15) can be cultured indefinitely and cloned readily. The RLA209-15 cells were temperature sensitive for maintenance of the transformed phenotype. These transformed liver cells selectively lost four characteristics of the transformed phenotype at the restrictive temperature (40 degrees C): generation time of the cells increased, the saturation density decreased, the efficiency of growth on nontransformed cell layers decreased, and the ability to clone in soft agar was lost. The transformation can be reversed simply by a shift in temperature. RLA209-15 fetal liver cells synthesized alpha-fetoprotein albumin, and transferrin. At 33 degrees C, the levels of these liver proteins were relatively low. At 40 degrees C the transformed phenotype was lost and the levels of alpha-fetoprotein, albumin, and transferrin were greatly increased. At the restrictive temperature, maximal induction of t...Continue Reading

References

Apr 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Y Chou
Mar 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J Y Chou
Apr 6, 1972·The New England Journal of Medicine·M A RothschildS S Schreiber
Apr 1, 1968·Biochemistry·F C Greene, R E Feeney
Jun 1, 1967·The Journal of Clinical Investigation·D Gitlin, M Boesman
May 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·S E Schlegel-HaueterJ Y Chou

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Citations

Dec 1, 1986·Journal of Bioenergetics and Biomembranes·I L SunH Löw
Mar 1, 1983·In Vitro·M A Feldman, J Y Chou
Nov 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·W H LamersR Charles
Apr 29, 1998·In Vitro Cellular & Developmental Biology. Animal·A S FiorinoL M Reid
Jan 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R H WhiteheadP S Jat
Feb 1, 1983·The Journal of Cell Biology·S P Banks-Schlegel, P M Howley
Jan 1, 1990·Critical Reviews in Biotechnology·C MacDonald
Apr 2, 2009·Biochimica Et Biophysica Acta·Patrizia BottoniRoberto Scatena
Jun 1, 1993·Trends in Biotechnology·J S McLean
Oct 14, 1983·Biochemical and Biophysical Research Communications·I L SunC Grebing
Oct 29, 1982·Biochemical and Biophysical Research Communications·J Y ChouM Feldman
Feb 1, 1984·Experimental and Molecular Pathology·J S ButelD Medina
Jan 1, 1983·Annals of the New York Academy of Sciences·J Y Chou, F Ito
Jan 13, 1984·Biochemical and Biophysical Research Communications·J Y Chou, F Ito
Jun 15, 1992·Biochemical and Biophysical Research Communications·T WatanabeJ Y Chou
Mar 28, 1986·Biochemical and Biophysical Research Communications·J Y Chou, A J Savitz
May 20, 1998·The Journal of Clinical Endocrinology and Metabolism·J HamM F Scanlon
Jan 1, 1988·Molecular and Cellular Biology·J Y ChouT Sakiyama
Aug 1, 1985·Biochimica Et Biophysica Acta·F L CraneH Löw

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