Study of microRNAs-21/221 as potential breast cancer biomarkers in Egyptian women

Gene
Tarek Mohamed Kamal MotawiFady Mohareb

Abstract

microRNAs (miRNAs) play an important role in cancer prognosis. They are small molecules, approximately 17-25 nucleotides in length, and their high stability in human serum supports their use as novel diagnostic biomarkers of cancer and other pathological conditions. In this study, we analyzed the expression patterns of miR-21 and miR-221 in the serum from a total of 100 Egyptian female subjects with breast cancer, fibroadenoma, and healthy control subjects. Using microarray-based expression profiling followed by real-time polymerase chain reaction validation, we compared the levels of the two circulating miRNAs in the serum of patients with breast cancer (n=50), fibroadenoma (n=25), and healthy controls (n=25). The miRNA SNORD68 was chosen as the housekeeping endogenous control. We found that the serum levels of miR-21 and miR-221 were significantly overexpressed in breast cancer patients compared to normal controls and fibroadenoma patients. Receiver Operating Characteristic (ROC) curve analysis revealed that miR-21 has greater potential in discriminating between breast cancer patients and the control group, while miR-221 has greater potential in discriminating between breast cancer and fibroadenoma patients. Classification mo...Continue Reading

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Citations

Sep 30, 2016·Artificial Cells, Nanomedicine, and Biotechnology·Wang JinlingWang Guinian
Aug 14, 2019·Cells·Dada Oluwaseyi TemilolaLuiz Fernando Zerbini
Jan 14, 2021·International Journal of Molecular Sciences·Hsing-Ju Wu, Pei-Yi Chu
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Sep 7, 2018·Genomics·Elmira Shajari, Hamidreza Mollasalehi
Aug 11, 2021·The International Journal of Biological Markers·Wenfeng LiuXin Yin

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