Study of mitotic chromatin supports a model of bookmarking by histone modifications and reveals nucleosome deposition patterns

Genome Research
Elisheva JavaskyItamar Simon

Abstract

Mitosis encompasses key molecular changes including chromatin condensation, nuclear envelope breakdown, and reduced transcription levels. Immediately after mitosis, the interphase chromatin structure is reestablished and transcription resumes. The reestablishment of the interphase chromatin is probably achieved by "bookmarking," i.e., the retention of at least partial information during mitosis. To gain a deeper understanding of the contribution of histone modifications to the mitotic bookmarking process, we merged proteomics, immunofluorescence, and ChIP-seq approaches. We focused on key histone modifications and employed HeLa-S3 cells as a model system. Generally, in spite of the general hypoacetylation observed during mitosis, we observed a global concordance between the genomic organization of histone modifications in interphase and mitosis, suggesting that the epigenomic landscape may serve as a component of the mitotic bookmarking process. Next, we investigated the nucleosome that enters nucleosome depleted regions (NDRs) during mitosis. We observed that in ∼60% of the NDRs, the entering nucleosome is distinct from the surrounding highly acetylated nucleosomes and appears to have either low levels of acetylation or high l...Continue Reading

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Citations

Jan 19, 2019·Genome Research·Nicola FestucciaPablo Navarro
Jan 30, 2020·Cell Cycle·M Lienhard SchmitzMarkus Seibert
Mar 20, 2019·Bioscience Reports·Shanshan LiuTianmin Xu
Apr 26, 2019·Archives of Pharmacal Research·Ja-Eun Kim
Jan 18, 2021·Current Opinion in Cell Biology·Inma GonzalezPablo Navarro
Oct 5, 2021·PLoS Computational Biology·Sergio SarnataroNacho Molina

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