Study of Polyphenol Content and Antioxidant Capacity of Myrianthus Arboreus (Cecropiaceae) Root Bark Extracts

Antioxidants
Pierre Betu KasanganaTatjana Stevanovic

Abstract

In order to evaluate the therapeutic potential of polyphenolic extracts from root bark of M. arboreus, we have determined the content of various polyphenols in aqueous and ethanol (EtOH) extract as well as two sub-fractions of the latter: ethyl acetate (EAc) and hexane (Hex). The total phenols, flavonoids, hydroxycinnamic acids and proanthocyanidins have been determined for all studied extracts/fractions by spectrophotometric methods. Both TP content (331.5 ± 2.5 mg GAE/g) and HCA content (201 ± 1.5 mg CAE/g) were determined to be the highest in EAc fraction of EtOH extract. All studied extracts were however determined to have a low content in flavonoids. The determination of antioxidant capacities of the studied extracts has also been performed by the following in vitro antioxidant tests: DPPH scavenging, phosphomolybdenum method and oxygen radical absorbance (ORACFl and ORACPRG) assay. The results of the DPPH free radical and ORACFl assays showed that there is no significant difference between the EAc fraction and Oligopin(®), but the EAc fraction exhibited the highest antioxidant capacity as determined by the phosphomolybdenium method. In addition, the EtOH extract was determined to have the same antioxidant efficiency as th...Continue Reading

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Citations

Nov 16, 2017·Biopolymers·Pritam RoySwagata Dasgupta
Mar 30, 2017·Journal of Basic and Clinical Physiology and Pharmacology·Oluwafemi Gabriel OluwoleSolomon Umukoro

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Methods Mentioned

BETA
Assay

Software Mentioned

Oligopin
Excel
GraphPad
GraphPad Prism

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