PMID: 6168443Jan 1, 1981Paper

Study of salivary gland specific transcripts in Chironomus tentans by cDNA analysis

Chromosoma
A Büchner-Vollmers, C P Hollenberg

Abstract

Preparative amounts of polyA-containing RNA were purified from isolated salivary glands from Chironomus tentans and used as a template for the synthesis of cDNA by AMV reverse transcriptase. The yield of cDNA corresponded to 8-14% of the input polyA RNA. Using the Alwine hybridization method (Alwine et al., 1977) it was shown that only a minor part of the cDNA hybridized to the 75S RNA band and that the major hybridization occurred to a hitherto unknown gland-specific polyA RNA transcript of 1.3 kb length. In addition, the cDNA hybridized to a gland-specific polyA-RNA transcript of 2 kb length. - The majority of the cDNA had a size of 0.4 or 0.7 kb in equal amounts. A Southern hybridization of the cDNA to an EcoRI digest of genomic DNA showed in addition to a very weak hybridization to the 40 kb band of the Balbiani ring genes, hybridization to several restriction fragments with sizes from 1.3 to 16 kb.

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·G K McMaster, G G Carmichael
Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·D J FinneganD S Hogness
May 5, 1978·Science·V B ReddyS M Weissman
Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·B DaneholtL Wieslander
Jan 1, 1978·Cold Spring Harbor Symposia on Quantitative Biology·J E EdströmL Rydlander
Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·J C AlwineG R Stark
Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·T ManiatisD G Kleid
Jan 1, 1975·Cell·B Daneholt
Jan 1, 1976·Analytical Biochemistry·J M Bailey, N Davidson
Jan 1, 1981·Chromosoma·A Degelmann, C P Hollenberg

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Jan 1, 1981·Chromosoma·A Degelmann, C P Hollenberg

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