Study of sample preparation for determination of endocannabinoids and analogous compounds in human serum by LC-MS/MS in MRM mode

Talanta
D Luque-CórdobaFeliciano Priego-Capote

Abstract

Endocannabinoids are lipids with a key role in physiological processes such as the immune response or the metabolism. This involvement explains their association to pathologies such as cancer, obesity or multiple sclerosis. The determination of endocannabinoids constitutes a challenge for clinical laboratories due to the variety of biological matrices and the wide range of concentrations at which they can be found. This research deals with the comparison of three sample preparation strategies (viz., on-line SPE, off-line SPE for interferents removal, and protein precipitation) for subsequent LC-MS/MS analysis of 14 endocannabinoids and analogous compounds in serum. As a result, the on-line coupling between SPE and LC-MS/MS is proposed as the best approach for this determination. The proposed method allows full automation of the overall process, shortening of the analysis time, and avoidance of errors associated with sample preparation steps. The improvement in sensitivity and selectivity thus achieved allows obtaining quantification limits at the pg mL-1 level, which makes possible the application of the method for clinical studies.

Citations

Sep 6, 2019·Bioanalysis·Momna AslamFlorin Marcel Musteata
Mar 4, 2020·Biomolecules·Iwona Jarocka-KarpowiczElżbieta Skrzydlewska
Oct 1, 2020·International Journal of Molecular Sciences·Olga SadowskaHanna Kozłowska
Nov 28, 2020·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Maria BobrichBurkhard Hinz

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