Abstract
To explore the postmortem physiological mechanism of muscle, activity of adenosine monophosphate activated protein kinase (AMPK) as well as its role in energy metabolism of postmortem yaks were studied. In this experiment, we injected 5-amino-1-beta-d-furanonyl imidazole-4-formamide (AICAR), a specific activator of AMPK, and STO-609 to observe the changes in glycolysis, energy metabolism, AMPK activity, and AMPK gene expression (PRKA1 and PRKA2) in postmortem yaks during maturation. The results showed that AICAR could increase the expression of the PRKKA1 and PRKAA2 genes, activate AMPK and increase its activity. The effects of AICAR include a lower concentration of ATP, an increase in AMP production, an acceleration of glycolysis, an increase in the lactic acid concentration, and a decrease in the pH value. In contrast, STO-609 had the opposite effect. Under hypoxic adaptation, the activity of the meat AMPK increased, which accelerated glycolysis and metabolism and more effectively regulated energy metabolism. Therefore, this study lays the foundation for establishing a theoretical system of energy metabolism in postmortem yak meat.
References
Nov 2, 1987·FEBS Letters·D CarlingD G Hardie
May 10, 1995·Archives of Biochemistry and Biophysics·K J RaserK K Wang
Apr 1, 1994·Current Biology : CB·J M CortonD G Hardie
Oct 1, 1996·Diabetologia·M F VincentG Van den Berghe
Jun 1, 1997·European Journal of Biochemistry·D G Hardie, D Carling
Oct 6, 2000·The Journal of Physiology·J F WojtaszewskiB Kiens
Apr 5, 2001·American Journal of Physiology. Endocrinology and Metabolism·N MusiL J Goodyear
Apr 28, 2001·Journal of Animal Science·E VeisethM Koohmaraie
Aug 18, 2001·Journal of Applied Physiology·W W Winder
May 17, 2002·Journal of Applied Physiology·S H ParkW W Winder
Jun 8, 2002·Biochemical and Biophysical Research Communications·Stephen R HamiltonLee A Witters
Jun 28, 2003·FEBS Letters·D Grahame HardieEmma R Hudson
Aug 28, 2003·Diabetes·Zhi-Ping ChenGlenn K McConell
Nov 15, 2003·Current Biology : CB·Angela WoodsDavid Carling
Jan 20, 2004·Trends in Biochemical Sciences·David Carling
Mar 19, 2004·The Journal of Biological Chemistry·Eun-Kyoung KimGabriele V Ronnett
Apr 3, 2004·Nature·Yasuhiko MinokoshiBarbara B Kahn
Aug 3, 2005·Molecular Cell·Morris J Birnbaum
Oct 1, 2005·Blood·Monika BrüggemannUNKNOWN German Multicenter Study Group for Adult Acute Lymphoblastic Leukemia
Oct 19, 2005·Journal of Animal Science·Q W ShenM Du
Dec 14, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Monika Proszkowiec-WeglarzJohn P McMurtry
Jul 20, 2006·Journal of Agricultural and Food Chemistry·Qingwu W ShenMin Du
Jan 5, 2007·Journal of Animal Science·Q W ShenM Du
Jan 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sharon M BlättlerUrs A Meyer
Apr 10, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·X FanR J McCrimmon
Sep 15, 2009·Biochemical and Biophysical Research Communications·Joseph W McFadden, Benjamin A Corl
Feb 24, 2011·The British Journal of Nutrition·Yongqing HouGuoyao Wu
Dec 1, 2002·Meat Science·X FernandezV Sante
Aug 1, 2006·Meat Science·T L CopenhaferD E Gerrard
Oct 1, 2006·Meat Science·Q W ShenM Du
Oct 1, 2005·Meat Science·Q W Shen, M Du
Mar 23, 2012·Nature Reviews. Molecular Cell Biology·D Grahame HardieSimon A Hawley