Study of the antidiabetic capacity of the VO(dmpp)2 complex

Journal of Inorganic Biochemistry
Marta PassadouroM Margarida C A Castro

Abstract

In this work we report biochemical ex vivo studies with a vanadium compound containing a pyridinone ligand, the bis(1,2-dimethyl-3-hydroxy-4-pyridinonate)oxovanadium (IV), V(IV)O(dmpp)(2), which has shown to have promising antidiabetic activity. The experiments were carried out on primary adipocytes of 6-8 week old Wistar rats. Insulin-stimulated glucose uptake studies were performed using a radioactive assay by measuring the (U)-(14)C-glucose taken up by the isolated adipocytes for 30 min. Adipocytes were incubated with and without insulin and in the presence and absence of different concentrations of V(IV)O(dmpp)(2) (100-500 microM) for 45 min. We observed that in a nontoxic concentration, as demonstrated by the Alamar Blue test, V(IV)O(dmpp)(2) significantly increases glucose uptake, in the absence of insulin, by 5-folds higher than basal, and it has a significant inhibitory effect of 78% on free fatty acid release in isolated adipocytes from normal rats. We also demonstrated that it promotes the phosphorylation of Akt1, a key protein in the insulin signaling cascade. These results were compared with those obtained with another vanadium compound reported in the literature, with a similar structure, the bis(maltolato)oxovanad...Continue Reading

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Citations

Apr 7, 2012·Analytical and Bioanalytical Chemistry·Björn Meermann, Michael Sperling
Dec 18, 2013·Journal of Inorganic Biochemistry·N DominguesM M C A Castro
Jul 13, 2014·Biological Trace Element Research·Mirosław KrośniakRyszard Gryboś
Apr 6, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Isabel CorreiaTamás Kiss
Jul 19, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Abeer Al-QatatiDebbie C Crans

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