PMID: 10001Sep 28, 1976

Study of the biological significance of cytochrome methylation. I. Thermal, acid and guanidinium hydrochloride denaturations of baker's yeast ferricytochromes c

Biochimica Et Biophysica Acta
E T PolastroJ Leonis

Abstract

The iso-cytochromes c from baker's yeast: iso-1 methylated and unmethylated forms and iso-2 have been purified and their stabilities towards denaturants compared to that of horse heart cytochrome c. Thermal, acid and guanidinium hydrochloride denaturations were followed using fluorescence emission of their tryptophan 59 and/or the absorbance in the Soret region as the physical parameters. Very few differences could be evidenced among the ferricytochromes investigated in this study insofar as the acid denaturations are concerned. This is to be contrasted with the conclusions of the thermal and guanidinium hydrochloride denaturations studies which clearly showed the ferricytochrome from horse heart to be much more stable than those from baker's yeast. No appreciable differences could be measured among the methylated and unmethylated forms of iso-1 cytochrome c nor among iso-1 and iso-2 cytochromes from baker's yeast. Our results suggest that a stabilizing effect of methylation on the tridimensional structure of ferricytochrome c must probably be discarded. Other possible physiological roles of methylation are suggested taking into account the relative instability of ascomycetes's cytochromes as compared to mammalian ones.

Citations

Nov 15, 1995·Molecular & General Genetics : MGG·D A Pearce, F Sherman
Aug 15, 1978·Experientia·Y R LoozeJ Léonis
Jan 1, 1978·The International Journal of Biochemistry·E T PolastroW K Paik
Jan 1, 1990·The International Journal of Biochemistry·B FrostW K Paik
Aug 21, 2013·Analytical Biochemistry·Ewelina GucaMariano Dellarole
Apr 26, 2013·Cellular and Molecular Life Sciences : CMLS·Sobia ZaidiFaizan Ahmad
Nov 15, 1988·Archives of Biochemistry and Biophysics·K S ParkW K Paik
Apr 7, 1992·Journal of Theoretical Biology·W K Paik, S Kim
Jul 11, 2012·Biochemistry·Gianantonio BattistuzziMarco Sola
Sep 15, 1983·European Journal of Biochemistry·W K PaikF Millett

Related Concepts

Metazoa
Cytochrome c Group
Guanidines
Hot Temperature
Hydrogen-Ion Concentration
Mathematics
Myocardium
Plasma Protein Binding Capacity
Protein Conformation
Protein Denaturation

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