Study of the cardiotoxicity of Venenum Bufonis in rats using an 1H NMR-based metabolomics approach

PloS One
Ge DongLingyi Kong

Abstract

Venenum Bufonis, a well-known traditional Chinese medicine, has been widely used in Asia and has gained popularity in Western countries over the last decade. Venenum Bufonis has obvious side effects that have been observed in clinical settings, but few studies have reported on its cardiotoxicity. In this work, the cardiotoxicity of Venenum Bufonis was investigated using a 11H NMR-based metabolomics approach. The 1H NMR profiles of the serum, myocardial extracts and liver extracts of specific-pathogen-free rats showed that Venenum Bufonis produced significant metabolic perturbations dose-dependently with a distinct time effect, peaking at 2 hr after dosing and attenuating gradually. Clinical chemistry, electrocardiographic recordings, and histopathological evaluation provided additional evidence of Venenum Bufonis-induced cardiac damage that complemented and supported the metabolomics findings. The combined results demonstrated that oxidative stress, mitochondrial dysfunction, and energy metabolism perturbations were associated with the cardiac damage that results from Venenum Bufonis.

References

Apr 1, 1977·Chemical & Pharmaceutical Bulletin·K ShimadaY Sato
Sep 1, 1992·Chest·T KwanL Kohl
Dec 1, 1967·Journal of Pharmaceutical Sciences·K K Chen, A Kovaríková
Sep 16, 1998·Natural Product Reports·P S Steyn, F R van Heerden
Sep 18, 1999·Free Radical Biology & Medicine·H Wang, J A Joseph
Jul 18, 2002·Nature Reviews. Drug Discovery·Jeremy K NicholsonElaine Holmes
May 6, 2003·Biochemical and Biophysical Research Communications·Jae-Sung KimJohn J Lemasters
Oct 29, 2003·Hypertension·Yoshihiro Taniyama, Kathy K Griendling
Sep 10, 2004·American Journal of Physiology. Cell Physiology·Paul S BrookesShey-Shing Sheu
Apr 19, 2005·Chemical Research in Toxicology·Nigel J WatersJeremy K Nicholson
Sep 7, 2006·Analytical and Bioanalytical Chemistry·Zhengzheng Pan, Daniel Raftery
Nov 23, 2006·Toxicology and Applied Pharmacology·Maria A ConstantinouEmmanuel Mikros
Mar 16, 2007·The New England Journal of Medicine·Stefan Neubauer
May 15, 2007·American Journal of Physiology. Cell Physiology·Wilhelm Schoner, Georgios Scheiner-Bobis
Jan 1, 2008·Toxicology and Applied Pharmacology·Lai WeiYijie Wu
Dec 15, 2010·Journal of Proteome Research·Juan LiHorst Joachim Schirra
Jun 24, 2011·Journal of Pharmaceutical and Biomedical Analysis·Yongmei HuWangfun Fong
Jun 1, 2012·Analytical and Bioanalytical Chemistry·Aihua ZhangXijun Wang
Sep 1, 2012·Amino Acids·Stephen W SchafferJunichi Azuma
Feb 21, 2013·Human & Experimental Toxicology·V AricaA Nacar

❮ Previous
Next ❯

Citations

Apr 5, 2016·Chemico-biological Interactions·Dan-Qian ChenYing-Yong Zhao
Oct 17, 2018·Environmental Toxicology and Chemistry·Jing FuBarry C Kelly
Apr 16, 2019·Rejuvenation Research·Si-Jun ZhaoXue-Mei Qin
Mar 3, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Dalinda Isabel Sánchez-VidañaMan-Sau Wong
Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Joaquin Lado-AbealMiguel López
Jun 21, 2016·The Journal of Veterinary Medical Science·Jiasan ZhengHongbin Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
nuclear magnetic resonance
Assay
Protein Assay
light microscopy
NMR
1
H
MDA
glycosylation

Software Mentioned

R
DA
noesypr1d
ARRIVE
OPLS
muma
Mestrelab
TOPSPIN
MestReNova

Related Concepts

Related Feeds

Bradyarrhythmias

Bradyarrhythmias are slow heart rates. Symptoms may include syncope, dizziness, fatigure, shortness of breath, and chest pains. Find the latest research on bradyarrhythmias here.

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.

ApoE, Lipids & Cholesterol

Serum cholesterol, triglycerides, apolipoprotein B (APOB)-containing lipoproteins (very low-density lipoprotein (VLDL), immediate-density lipoprotein (IDL), and low-density lipoprotein (LDL), lipoprotein A (LPA)) and the total cholesterol/high-density lipoprotein (HDL) cholesterol ratio are all connected in diseases. Here is the latest research.

Cardiotoxicity

Cardiotoxicity refers to the muscle damage or dysfunction of heart electrophysiology caused by drug intake or due to disease complications. It is a well-known side effect of several cytotoxic drugs, especially of the anthracyclines and can lead to long term morbidity. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis