PMID: 8582051Oct 1, 1995Paper

Study of the heterogeneity of creatine kinase-MM isoforms using western blotting

Clinical Biochemistry
I Ramasamy

Abstract

To study creatine kinase isoform variants in muscle (skeletal and myocardial) damage using a Western blotting procedure. The study comprised of 16 patients admitted with chest pain, 14 patients with skeletal muscle damage, and 4 healthy individuals. The creatine kinase-MM (CK-MM) isoforms were separated by isoelectric focusing and detected by electrophoretic transfer to a nitrocellulose membrane and immunoblotting. Binding of the first monoclonal mouse anti-human CK-MM antibody was detected by either a horseradish peroxidase or alkaline phosphatase anti-mouse immunoglobulin conjugate. Three major variants of CK-MM (MM3 pl = 6.8; MM2 pl = 6.4; MM1 pl = 6.2) were detected. Minor sub-bands were detected in 4/16 and 3/14 patients with acute myocardial infarction (AMI) and skeletal muscle damage, respectively. The tissue form (MM0 pl = 7.14) was visible in 6/16 patients with AMI and 4/14 patients with skeletal muscle damage. The appearance of minor variants was therefore of limited diagnostic value in discriminating between patients with and without AMI. Immunoblotting confirms the parallel increase in mass and activity of CK-MM isoforms, thus confirming measurement of isoform activity as an early marker of AMI or reperfusion follow...Continue Reading

References

Jun 15, 1978·Clinica Chimica Acta; International Journal of Clinical Chemistry·R A WeversJ B Soons
Mar 15, 1977·Clinica Chimica Acta; International Journal of Clinical Chemistry·R A WeversJ B Soons
Aug 1, 1988·Clinical Biochemistry·M Panteghini
Mar 1, 1987·The American Journal of the Medical Sciences·R L MorelliE Rapaport
Jun 1, 1987·Scandinavian Journal of Clinical and Laboratory Investigation·M Panteghini, M Calarco
Nov 1, 1986·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·E SiragEldinK Harm
Jun 14, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·R A WeversJ B Soons
Jan 1, 1983·Annual Review of Biochemistry·A Meister, M E Anderson
Jan 1, 1994·Journal of Clinical Laboratory Analysis·Y UjiM Miura

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.