PMID: 11931971Apr 5, 2002Paper

Study of the influence of metal ions on tRNA(Phe) thermal unfolding equilibria by UV spectroscopy and multivariate curve resolution

Journal of Inorganic Biochemistry
M VivesR Gargallo

Abstract

The influence of metal ions (Na(+), Mg(2+) and Cd(2+)) on the thermal unfolding of phenylalanine transfer ribonucleic acid (tRNA(Phe)) was studied by UV spectroscopy-monitored melting experiments. Absorbance data were obtained during the unfolding process in the range 220-340 nm and later analyzed by a multivariate curve resolution approach (MCR-ALS) based on factor analysis. This procedure determines the number of spectroscopically distinct conformations present during the unfolding process and reveals their concentration profiles and pure spectra, without any initial assumption having to be made about the number of steps in the unfolding pathway. From the concentration profiles and pure spectra, information such as T(m) values can be recovered. The results were compared with those obtained previously in spectroscopic and calorimetric unfolding experiments, showing that the multivariate approach recovers information that complements that obtained in traditional spectroscopic melting experiments.

References

Jul 15, 1978·Journal of Molecular Biology·P L Privalov, V V Filimonov

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Citations

Jun 7, 2011·Biophysical Journal·Thomas R Einert, Roland R Netz
Apr 3, 2021·International Journal of Biological Macromolecules·Ali R Jalalvand
Nov 25, 2003·Journal of Chemical Information and Computer Sciences·L DuponchelJ P Huvenne

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