PMID: 7357001Feb 29, 1980Paper

Study of the interaction of glyceraldehyde-3-phosphate dehydrogenase with DNA

Biochimica Et Biophysica Acta
M PeruchoM L Salas


Glyceraldehyde-3-phosphate dehydrogenase binds to homologous and heterologous single-stranded but not double-stranded DNA. Binding to RNA, poly(A) and poly(dA-dT) has also been observed. Enzyme binding to these nucleic acids leads to the formation of an insoluble complex which can be sedimented at low speed. The interaction of glyceraldehyde-3-phosphate dehydrogenase with DNA is strongly inhibited by NAD and NADH but not by NADP. Adenine nucleotides, which inhibit the dehydrogenase activity by competing with NAD for its binding site (Yang, S.T. and Deal, W.C., Jr. (1969) Biochemistry 8, 2806--2813), also inhibit enzyme binding to DNA, whereas glyceraldehyde-3-phosphate and inorganic phosphate are non-inhibitory. These results suggest that DNA interacts through the NAD binding sites of glyceraldehyde-3-phosphate dehydrogenase. In accordance with this idea, it was found that DNA also binds to lactate dehydrogenase, an enzyme containing a similar dinucleotide binding domain, and that this binding is inhibited by NADH. A study of the base specificity of the DNA-glyceraldehyde-3-phosphate dehydrogenase interaction using dinucleoside monophosphates shows that inhibition of DNA binding by the dinucleotides requires the presence of a 3...Continue Reading


Jun 1, 1995·Journal of Molecular Evolution·N C Kyrpides, C A Ouzounis
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H RübsamenE Eigenbrodt
Sep 12, 2013·BioMed Research International·Zaira LeniAlexandre Arcaro
Jan 1, 1982·Progress in Biophysics and Molecular Biology·C Hélène, G Lancelot
Nov 18, 2014·Biochemical Society Transactions·Brian Henderson, Andrew C R Martin
May 1, 1994·Current Eye Research·A EspaillatR B Colvin
Mar 9, 2005·Trends in Biochemical Sciences·Jung-Whan Kim, Chi V Dang
May 25, 2016·International Journal of Biological Macromolecules·Vladimir I MuronetzElena V Schmalhausen
Jun 15, 1989·Biochemical and Biophysical Research Communications·V V FilonenkoL L Kisselev
Sep 1, 1994·Scandinavian Journal of Immunology·V Arrunategui-CorreaC S Foster
Oct 1, 1989·Journal of Cellular Physiology·D L Stoler, K F Manly
Jan 15, 1988·European Journal of Biochemistry·A G RyazanovV I Muronetz
Aug 8, 2006·Oncogene·H PelicanoP Huang
Dec 3, 2014·The Journal of Biological Chemistry·Michael R WhiteElsa D Garcin
Jan 1, 1993·Progress in Biophysics and Molecular Biology·L A Fothergill-Gilmore, P A Michels

❮ Previous
Next ❯

Related Concepts

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.


Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.


Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.