Study of the interaction of trastuzumab and SKOV3 epithelial cancer cells using a quartz crystal microbalance sensor

Sensors
Louise ElmlundIan A Nicholls

Abstract

Analytical methods founded upon whole cell-based assays are of importance in early stage drug development and in fundamental studies of biomolecular recognition. Here we have studied the binding of the monoclonal antibody trastuzumab to human epidermal growth factor receptor 2 (HER2) on human ovary adenocarcinoma epithelial cancer cells (SKOV3) using quartz crystal microbalance (QCM) technology. An optimized procedure for immobilizing the cells on the chip surface was established with respect to fixation procedure and seeding density. Trastuzumab binding to the cell decorated sensor surface was studied, revealing a mean dissociation constant, KD, value of 7 ± 1 nM (standard error of the mean). This study provides a new perspective on the affinity of the antibody-receptor complex presented a more natural context compared to purified receptors. These results demonstrate the potential for using whole cell-based QCM assay in drug development, the screening of HER2 selective antibody-based drug candidates, and for the study of biomolecular recognition. This real time, label free approach for studying interactions with target receptors present in their natural environment afforded sensitive and detailed kinetic information about the ...Continue Reading

References

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Citations

Dec 21, 2016·Journal of Molecular Recognition : JMR·Martin PeslVladimir Rotrekl
May 30, 2019·Biosensors·Alexandra PoturnayováTibor Hianik
Apr 9, 2020·Journal of Hematology & Oncology·Juan Manuel DomínguezJuan M Zapata
Jun 30, 2016·Advanced Drug Delivery Reviews·Hyun Jin KimKazunori Kataoka
Jun 27, 2019·Biosensors & Bioelectronics·Niharika GuptaBansi D Malhotra

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Methods Mentioned

BETA
flow cytometry
biosensors
quartz
biosensor
quartz crystal microbalance
chip
chips
fluorescence microscopy
Fluorescence
ELISA

Software Mentioned

Attester
Attana

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