Study of the intercellular fluid of healthy Lupinus albus organs. Presence of a chitinase and a thaumatin-like protein

Plant Physiology
A P Regalado, C P Ricardo

Abstract

Proteins in the intercellular fluid (IF) of healthy Lupinus albus leaves were characterized. Silver staining of the proteins separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis revealed more than 30 polypeptides, with the major ones having a molecular mass lower than 36 kD. After amino-terminal amino acid sequence analysis, one of the major polypeptides, IF4, was shown to have no identity with any of the proteins present in the data bases. Two others, IF1 and IF3, showed identity with previously reported pathogenesis-related proteins, IF1 with an antifungal protein from Hordeum vulgare that belongs to the thaumatin family (PR-5 family), and IF3 with class III chitinase-lysozymes. IF3 was also present in the IF of stem and root and it represents the major polypeptide in the medium of L. albus cell-suspension cultures. The ubiquitous presence of this enzyme in healthy, nonstressed tissues of L. albus cannot be explained.

References

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Citations

Apr 14, 2009·Journal of Experimental Botany·Konstantin Yu KulichikhinTimothy D Colmer
Sep 18, 2010·Journal of Experimental Botany·Timothy David Colmer, Hank Greenway
Aug 26, 1998·Proceedings of the National Academy of Sciences of the United States of America·A W SowaR D Hill
Sep 1, 2007·Molecular Plant Pathology·Ricardo B FerreiraArtur R Teixeira
May 15, 2015·Applied Microbiology and Biotechnology·Juan YanZu-Jian Wu
Aug 11, 2000·Journal of Experimental Botany·S HelleboidJ L Hilbert
Nov 13, 2001·The Journal of Biological Chemistry·Silvio ArpagausRoland Braendle
Aug 15, 2021·International Journal of Biological Macromolecules·Robert NawrotAnna Goździcka-Józefiak

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