Study of the irreversible binding of Bacillus thuringiensis Cry1Aa to brush border membrane vesicles from Bombyx mori midgut

Journal of Invertebrate Pathology
Hideshi Ihara, Michio Himeno

Abstract

The binding of Bacillus thuringiensis delta-endotoxin to brush border membrane vesicles (BBMVs) from the target insect larval midgut comprises with not only a reversible but also an irreversible component. The irreversible binding of delta-endotoxin is thought to be a pathologically important factor. Here, we studied the irreversible binding of Cry1Aa to the BBMVs of Bombyx mori. The (125)I-labeled Cry1Aa bound to the solubilized brush border membrane (BBM) through rapid dissociation only, unlike the binding to BBMVs, indicating that the toxin bound to the solubilized BBM through only a reversible process. Low-temperature sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed that the toxin bound irreversibly to BBMVs formed an oligomer of 220kDa, whereas that bound reversibly to the solubilized BBM did not oligomeraize. When the (125)I-labeled Cry1Aa bound irreversibly to the BBMVs was digested by proteinase K, approximately 40% of the toxin observed to be resistant to proteinase K. The molecular mass of the toxin resistant to proteinase K was 60kDa, suggesting that the irreversible binding comprise two forms. These results support the notion that the irreversible binding of the toxin to BBMVs is due to th...Continue Reading

References

Dec 1, 1995·Journal of Molecular Biology·P GrochulskiM Cygler
Mar 10, 1995·The Journal of Biological Chemistry·R K VadlamudiL A Bulla
Jun 6, 1998·Bioscience, Biotechnology, and Biochemistry·Y NagamatsuT Katsumoto
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·H IharaM Himeno
May 29, 1999·Applied and Environmental Microbiology·A I AronsonL Wu
Jul 27, 2001·Acta Crystallographica. Section D, Biological Crystallography·N GalitskyL English
Apr 7, 2005·Journal of Molecular Biology·Panadda BoonsermJade Li
Jun 30, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Kazuya TomimotoHidetaka Hori
Jan 3, 2007·Toxicon : Official Journal of the International Society on Toxinology·Alejandra BravoMario Soberón
Jun 1, 2007·The Journal of Biological Chemistry·Nuria Jiménez-JuárezAlejandra Bravo
Jun 5, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Masahiro HiguchiHidetaka Hori
Jan 1, 1993·Bioscience, Biotechnology, and Biochemistry·H IharaM Himeno

❮ Previous
Next ❯

Citations

May 24, 2012·Journal of Invertebrate Pathology·Vincent VachonJean-Louis Schwartz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.