Study of the maturation of the organic (type I collagen) and mineral (nonstoichiometric apatite) constituents of a calcified tissue (dentin) as a function of location: a Fourier transform infrared microspectroscopic investigation

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
D MagneG Daculsi

Abstract

Fourier transform infrared microspectroscopy (FTIRM) was used to investigate the organic and mineral phases of a calcified tissue (dentin) as a function of its location from predentin toward enamel. Thin dentin slices (decalcified or not) were fixed in formaldehyde and embedded in glycolmethylmethacrylate (GMA). Fixation did not denature collagen, and GMA did not interact with organic or mineral constituents of dentin. The v1v3 PO4 domain was studied in particular in order to estimate mineral maturity and amide I, II, A, and B to obtain data on protein conformation. The results showed that dentin apatite became increasingly mature (stoichiometric) from the mineralization front toward the enamel, especially through loss of HPO4(2-) groups and vacancies. Moreover, collagen fibrils became less and less hydrated, suggesting that intrafibrillar mineralization partially dehydrated the collagen. Combined study of the organic and mineral fractions of calcified tissues may help clarify their relationships in physiological and pathological tissues.

References

Jun 28, 1977·Biochemistry·S L LeeT Glonek
Feb 1, 1977·Biopolymers·S NomuraE Baer
May 1, 1975·Biopolymers·B B DoyleE R Blout
Jul 1, 1992·Calcified Tissue International·N L PleshkoR Mendelsohn
Jun 1, 1992·Matrix : Collagen and Related Research·W TraubB Sabsay
Sep 1, 1992·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·N L PleshkoR Mendelsohn
Feb 1, 1992·Biopolymers·Y A LazarevV S Grechishko
Jan 1, 1991·Acta Paediatrica Scandinavica. Supplement·D J Prockop
Feb 1, 1991·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·J T Mason, T J O'Leary
Apr 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·H KuivaniemiD J Prockop
Oct 1, 1986·Journal of Oral Pathology·Y Takagi, S Sasaki
Jan 1, 1989·Connective Tissue Research·E P KatzG L Mechanic
Jan 20, 1985·Journal of Molecular Biology·L C BonarH A Mook
Jan 26, 1972·Biochimica Et Biophysica Acta·W T ButlerC V Desteno
Jul 1, 1982·Calcified Tissue International·A L BoskeyP G Bullough
Sep 1, 1981·Journal of Dental Research·B KerebelL M Kerebel
Oct 1, 1995·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C ReyM J Glimcher
Apr 1, 1996·Archives of Oral Biology·R M CarvalhoD H Pashley
Dec 1, 1996·Calcified Tissue International·E P PaschalisA L Boskey
Feb 12, 1998·Calcified Tissue International·E P PaschalisA L Boskey
Apr 16, 1998·European Journal of Oral Sciences·R Fujisawa, Y Kuboki
Jul 9, 1998·Free Radical Biology & Medicine·S M LeVine, D L Wetzel
Oct 1, 1961·Journal of Molecular Biology·A RICH, F H CRICK

❮ Previous
Next ❯

Citations

Mar 5, 2010·Journal of Dental Research·J KimF R Tay
Jul 28, 2010·Journal of Dental Research·E OsorioR Osorio
Oct 14, 2010·Journal of Dental Research·F T SadekF R Tay
Oct 14, 2010·Journal of Dental Research·L GuF R Tay
Mar 15, 2005·Connective Tissue Research·D MagneJ Guicheux
Oct 18, 2014·Clinical Oral Investigations·Tamires T MaskeRafael R Moraes
Feb 10, 2007·Bone·K VerdelisA L Boskey
Apr 25, 2007·Journal of Dental Research·H K YipW H S Wong
Oct 26, 2007·Journal of Dental Research·T JiangJ Hu
Dec 18, 2012·Journal of Dental Research·C SatoI L S Tersariol
Apr 12, 2002·Journal of Biomedical Materials Research·I KhairounP Weiss
Oct 19, 2005·Journal of Biomedical Optics·Nicole J CraneGenggeng Yu
Nov 24, 2004·Calcified Tissue International·Yahya AçilIngo Springer
Oct 20, 2020·Advanced Healthcare Materials·Rachel L WilmothStephanie J Bryant
Apr 14, 2021·Dental Materials : Official Publication of the Academy of Dental Materials·Manuel ToledanoRaquel Osorio

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.