Study of the mechanism underlying the inhibitory effects of transglutaminase II on apoptosis in the osteosarcoma MG-63 cell line under hypoxic conditions

Oncology Letters
Guobin WangFangmin Chen

Abstract

The aim of the present study was to investigate the association between the apoptosis phenomenon in the MG-63 osteosarcoma cell line, and transglutaminase II (TG2) expression. The relationship between the anti-apoptotic mechanism of TG2 and the expression of cytochrome c as well as caspase-3 under hypoxic conditions was also verified. A hypoxic culture of MG-63 cells was prepared. The hypoxia and TG2 siRNA hypoxia groups were established, and the cultures were incubated for 12 h under hypoxic conditions. TG2 activity, TG2 protein expression and its mRNA level were investigated. Cytochrome c and caspase-3 protein levels in the TG2 nucleus and cytoplasm were measured. The apoptotic rate was also monitored. The results showed that TG2 activity, TG2 protein expression and its mRNA level in the hypoxia group were significantly higher than those of the siRNA hypoxia group. The results showed statistically insignificant differences (P<0.05). By contrast, a comparison of the two groups in the cytoplasm yielded no statistically significant differences (P>0.05). Cytochrome c and caspase-3 protein levels in the hypoxia group were significantly higher than those of the TG2 siRNA hypoxia group. The results showed statistically significant d...Continue Reading

References

Jun 9, 2001·Methods in Cell Biology·N J WaterhouseD R Green
Jun 10, 2005·FEBS Letters·László Fésüs, Zsuzsa Szondy
Oct 22, 2005·Nature Reviews. Cancer·Stephen W Fesik
Feb 29, 2008·BMB Reports·Seon-Yong Jeong, Dai-Wu Seol
Jul 4, 2008·Amino Acids·Angelo Facchiano, Francesco Facchiano
Aug 3, 2010·Experimental & Molecular Medicine·Sung-Yup ChoIn-Gyu Kim
Aug 2, 2011·Amino Acids·Daniela CaccamoRiccardo Ientile
Mar 1, 2012·International Review of Cell and Molecular Biology·Maria V Nurminskaya, Alexey M Belkin
May 25, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Bo Mi KuSoo-Youl Kim

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Methods Mentioned

BETA
transfection
PCR
electrophoresis

Software Mentioned

SPSS

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis