Study of the Metatranscriptome of Eight Social and Solitary Wild Bee Species Reveals Novel Viruses and Bee Parasites

Frontiers in Microbiology
Karel SchoonvaereDirk C de Graaf

Abstract

Bees are associated with a remarkable diversity of microorganisms, including unicellular parasites, bacteria, fungi, and viruses. The application of next-generation sequencing approaches enables the identification of this rich species composition as well as the discovery of previously unknown associations. Using high-throughput polyadenylated ribonucleic acid (RNA) sequencing, we investigated the metatranscriptome of eight wild bee species (Andrena cineraria, Andrena fulva, Andrena haemorrhoa, Bombus terrestris, Bombus cryptarum, Bombus pascuorum, Osmia bicornis, andOsmia cornuta) sampled from four different localities in Belgium. Across the RNA sequencing libraries, 88-99% of the taxonomically informative reads were of the host transcriptome. Four viruses with homology to insect pathogens were found including two RNA viruses (belonging to the familiesIflaviridaeandTymoviridaethat harbor already viruses of honey bees), a double stranded DNA virus (familyNudiviridae) and a single stranded DNA virus (familyParvoviridae). In addition, we found genomic sequences of 11 unclassified arthropod viruses (related to negeviruses, sobemoviruses, totiviruses, rhabdoviruses, and mononegaviruses), seven plant pathogenic viruses, and one funga...Continue Reading

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May 12, 2018·The Journal of General Virology·John M K RobertsPeter A Durr
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Aug 4, 2019·Insects·Joseph Belsky, Neelendra K Joshi
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Aug 7, 2021·Insects·Alexander J McMenaminMichelle L Flenniken
Nov 12, 2021·Archives of Virology·Xiao-Di ZhangJun-Min Li

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Datasets Mentioned

BETA
PRJNA411946

Methods Mentioned

BETA
RNA-Seq
cDNA library
PCRs
PCR

Software Mentioned

CLC Genomics Workbench
HISAT2
BLAST +
DIAMOND
ggplot2
Trinity
R scripts
Interproscan
PhyML
ProtTest3

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