Study of the nature of recognition in molecularly imprinted polymers

Journal of Molecular Recognition : JMR
H S AnderssonK Mosbach

Abstract

In the present study molecularly imprinted polymers (MIPs) were prepared against a series of structurally related compounds containing various numbers of pyridyl groups. The goal, to increase understanding of the mechanisms of recognition in MIPs, was achieved by comparing the patterns of retention of the imprinted compounds on the different MIPs when related to a blank (non-imprinted) polymer in a high performance liquid chromatography system. Furthermore, frontal analysis was carried out on three polymers: a blank, a pyridine-imprinted and a 4,4'-bipyridyl-imprinted polymer, to evaluate the number (Bt), average specificity and strength (dissociation constant; Kdiss) of the recognition sites. The Kdiss values of pyridine on the different polymers were in the range 0.10-0.12 M, and the amount of imprinted binding sites (Bt) 0.10-0.12 mmol/g. Kdiss values of 4,4'-bipyridyl were approximately 0.06 M, with Bt values equal to the above, except for in the anti-4,4'-bipyridyl polymer where the Kdiss was determined to be 0.02 M and Bt 0.07 mmol/g. From the results it can be concluded that multiple additive weak interactions dominate the recognition of the template molecules in these imprinted polymers.

References

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Citations

Mar 17, 1999·Journal of Molecular Recognition : JMR·H S Andersson, O Ramström
Mar 17, 1999·Journal of Molecular Recognition : JMR·I A Nicholls
Jan 1, 2008·Analytical and Bioanalytical Chemistry·Wen-Chien LeeChing-Chiang Hwang
Feb 24, 2001·Journal of Chromatography. a·B Sellergren
May 2, 2002·Biochimica Et Biophysica Acta·Pierre Lemay
Jun 11, 2009·The Journal of Chemical Physics·Eduardo M A Dourado, Lev Sarkisov
Mar 14, 2008·Analytical Chemistry·Feliciano Priego-CapoteStaffan Nilsson
Sep 29, 2011·International Journal of Molecular Sciences·Eduardo M A DouradoLev Sarkisov
Mar 17, 1999·Journal of Molecular Recognition : JMR·M C NorellI A Nicholls
Jul 27, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Luis Enrique Gómez-PinedaMaría de Los Angeles Cuán Hernández
Oct 10, 2015·Soft Matter·Tine CurkDaan Frenkel
Feb 28, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Carmelo Herdes, Lev Sarkisov
May 23, 2001·Journal of Agricultural and Food Chemistry·O RamströmO Brüggemann
Jul 18, 2001·Journal of the American Chemical Society·M QuagliaB Sellergren

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