Study of the Potential Radiomitigator Effect of Quercetin on Human Lymphocytes

Inflammation
Williams Nascimento de SiqueiraAna Maria Mendonça de Albuquerque Melo

Abstract

Several substances of synthetic and natural origin have been studied to determine their ability to protect the body from damage caused by ionizing radiation. Among these substances, quercetin has been shown to be a naturally occurring molecule with high radioprotective and radiomitigator potential due to its antioxidant properties. The objective of this work was to ascertain the potential radiomitigator effect of quercetin on chromosome aberration yield in lymphocytes of in vitro-irradiated human peripheral blood. At first, the DPPH (2,2-diphenyl-1-picryl-hydrazyl) radical capture test was performed to determine the antioxidant activity of quercetin and to select the concentrations to be tested. The blood was irradiated at doses of 2.5, 3.5, and 4.5 Gy and lymphocytes were cultured with quercetin at preselected concentrations of 37.5 and 75 μM. Then, the slides were prepared for scoring unstable chromosome aberrations (dicentrics, rings, and fragments). The results showed that the lymphocytes irradiated and later exposed to quercetin presented a lower frequency of chromosomal alterations compared to the control sample which was irradiated and not exposed to quercetin. The results suggest a potential radiomitigator effect of the...Continue Reading

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Citations

Dec 29, 2020·Problemy Radiat︠s︡iĭnoï Medyt︠s︡yny Ta Radiobiolohiï·G N VoitenkoO G Lutsenko
Sep 30, 2021·International Journal of Radiation Biology·Mariana Brayner CavalcantiEdvane Borges da Silva

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Methods Mentioned

BETA
X-ray
chromosomal aberrations
PMA
enzyme-linked immunosorbent assay
ELISA
Assay

Software Mentioned

GraphPad Prism

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