Study of the reductive dechlorination of pentachlorophenol by a methanogenic consortium

Canadian Journal of Microbiology
P JuteauJ G Bisaillon

Abstract

Pentachlorophenol (PCP) dechlorination by a methanogenic consortium was observed when glucose, formate, lactate, or yeast extract was present in the mineral medium as a secondary carbon source. Acetate was not a good substrate to sustain dechlorination. The consortium was able to dechlorinate the different monochlorophenols, although the chlorine in position ortho and meta was removed more readily than in para position. Dechlorination was most efficient at 37 degrees C. At 45 degrees C, the first PCP dechlorination steps were very rapid, but 3,5-dichlorophenol (3,5-DCP) was not further dechlorinated. At 15 and 4 degrees C, dechlorination was very slow. The dechlorination of PCP to 3-chlorophenol (3-CP) was still observed after the consortium had been subjected to heat treatment (80 degrees C, 60 min), suggesting that spore-forming bacteria were responsible. The dechlorinating activity of the consortium was significantly reduced by the presence of hydrogen, 2-bromoethanosulfonic acid (BESA), or sulfate but not of nitrate. The dechlorination of 3-CP was completely inhibited by heat treatment or the presence of BESA, suggesting that a syntrophic microorganism would be involved. Vigorous agitation of the consortium stopped the dech...Continue Reading

References

Sep 1, 1992·FEMS Microbiology Reviews·M M Häggblom
Sep 1, 1992·Applied and Environmental Microbiology·T Madsen, D Licht
Jan 1, 1992·Applied and Environmental Microbiology·H V HendriksenB K Ahring
Apr 1, 1992·Applied and Environmental Microbiology·W W Mohn, K J Kennedy
Feb 1, 1992·Applied and Environmental Microbiology·T Madsen, H Aamand
Jul 1, 1992·Applied and Environmental Microbiology·W W Mohn, K J Kennedy
Apr 1, 1990·Applied and Environmental Microbiology·X Zhang, J Wiegel
Jul 1, 1983·Applied and Environmental Microbiology·S A BoydJ M Tiedje
Feb 1, 1984·Applied and Environmental Microbiology·S A Boyd, D R Shelton
Jun 1, 1989·Applied and Environmental Microbiology·B R GenthnerP H Pritchard
Sep 1, 1991·Applied and Environmental Microbiology·T Madsen, J Aamand

❮ Previous
Next ❯

Citations

Mar 13, 2013·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Richard Villemur
Aug 17, 2006·FEMS Microbiology Reviews·Richard VillemurFrançois Lépine
Oct 31, 1998·Applied and Environmental Microbiology·D DennieR Beaudet

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.