PMID: 7025889Jul 7, 1981Paper

Study of transfer ribonucleic acid unfolding by dynamic nuclear magnetic resonance

Biochemistry
P D Johnston, A G Redfield

Abstract

Nuclear magnetic resonance (NMR) measurements of proton exchange were performed on yeast tRNAPhe, and in much less detail on Escherichia coli tRNAfMet, over a range of Mg2+ concentrations and temperatures, at neutral pH and 0.1 M NaCl. The resonances studied were those of ring nitrogen protons, resonating between 10 and 15 ppm downfield from sodium 3-(trimethylsilyl)-1-propanesulfonate, which partake in hydrogen bonding between bases of secondary and tertiary pairs. Methods include saturation--recovery, line width, and real-time observation after a change to deuterated solvent. The relevant theory is briefly reviewed. We believe that most of the higher temperature rates reflect major unfolding of the molecule. For E. coli tRNAfMet, the temperature dependence of the rate for the U8--A14 resonance maps well onto previous optical T-jump studies for a transition assigned to tertiary melting. For yeast tRNAPhe, exchange rates of several resolved protons could be studied from 30 to 45 degrees C in zero Mg2+ concentration and had activation energies on the order of 40 kcal/mol. Initially, the tertiary structure melts, followed shortly by the acceptor stem. At high Mg2+ concentration, relatively few exchange rates are measurable below ...Continue Reading

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Citations

Jul 13, 1988·Biochimica Et Biophysica Acta·N FosterK J Schray
Aug 1, 1987·Quarterly Reviews of Biophysics·D J PatelD Hare
Nov 1, 1983·Quarterly Reviews of Biophysics·S W Englander, N R Kallenbach
Apr 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·D J PatelK Itakura
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S CheungP Lu
Dec 1, 1985·Journal of Biomolecular Structure & Dynamics·T PielerV A Erdmann
Nov 11, 1982·Nucleic Acids Research·H RüterjansH H Limbach
Apr 11, 1983·Nucleic Acids Research·J S Tropp, A G Redfield
Aug 15, 1997·Nucleic Acids Research·E J Maglott, G D Glick
Dec 1, 1988·European Journal of Biochemistry·F J Van de Ven, C W Hilbers
May 17, 1982·FEBS Letters·E KaunW E Hull
Jul 12, 2011·Journal of Molecular Biology·Lucas G Nivón, Eugene I Shakhnovich
Apr 16, 1985·Biochemical and Biophysical Research Communications·P Bendel
Dec 29, 1992·Biochemistry·B S Choi, A G Redfield
Apr 8, 1986·Biochemistry·B S Choi, A G Redfield
May 7, 1982·Science·D J PatelK Itakura

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