PMID: 11917845Mar 29, 2002Paper

Study on apoptosis of human gastric adenocarcinoma cell SGC-7901 induced by aining in vitro and the cell cycle

Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials
M SuJ Liu

Abstract

To study the inducing apoptosis effect of Aining on human gastric adenocarcinoma cell in vitro. Agarose gel electrophoresis (AGE) and flow cytometry (FMC) techniques were adopted to study the inducing apoptosis effect of Ainging on the human gastric adenocarcinoma cell. The human gastric adenocarcinoma cell's DNA was digested into fragments which gave rise to a characteristic "DNA Ladder" on agarose gel electrophoresis, while the percentage of apoptotic cells was 43.5%. But the blank group had neither DNA ladder, nor apoptotic peak. Cell cycle analysis showed that the proliferation index was 33.7% after the human gastric adenocarcinoma cells treated with Aining, and there was no cell phase specialty. Aining can induce human gastric adenocarcinoma cell apoptosis in vitro.

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

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