PMID: 11324521Apr 28, 2001Paper

Study on delayed rectifier K+ current of rabbit vascular smooth muscle cells and comparison with cloned Kv1.5 channel

Sheng li xue bao : [Acta physiologica Sinica]
W H XuX L Wang

Abstract

Whole cell patch-clamp recording techniques were used to compare the electrophysiological properties between delayed rectifier K+ current of rabbit vascular smooth muscle cell (VSMC) and the cloned Kv1.5 channel. When VSMC is clamped at -40 mV, depolarizing the membrane potential at increasing steps of 10 mV could evoke a series of outward potassium currents without any deactivation. The V1/2 of the activation curve was 27.2 mV. The currents decrease obviously after adding 100 mmol/L TEA or 1 mmol/L 4AP in the perfusate. When the concentration of extracellular Ca2+ was decreased from 1.5 mmol/L to 0.5 or 0 mmol/L, the currents did not show much change, while in HBK7 (cloned Kv1.5 channel cell) held at -80 mV, similar steparise depolarization could also produce a series of outward potassium currents without any deactivation decayed. V1/2 of the activation was 0.8 mV. 4AP inhibited the cloned channel current with IC50 of 7.3 mmol/L, showing neither frequency- or use-dependence. TEA (30, 100 and 300 mmol/L) reduced the current by 28.6%, 37.4% and 46.3% respectively. Quinidine (0.1 and 1 mmol/L) decreased it by 29.7% and 37.4%. These results show what we have recorded in isolated rabbit vascular smooth muscle cells is the delayed r...Continue Reading

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