Study on fluorescence properties of HCV antiviral (velpatasvir) and its fluorimetric determination in presence of sofosbuvir; application to stability study and human plasma

Luminescence : the Journal of Biological and Chemical Luminescence
Mahmoud A OmarSayed M Derayea

Abstract

Velpatasvir (VLP) is a new, oral, direct-acting antiviral with potent inhibitory activity against all hepatitis C virus (HCV) genotypes. A highly sensitive, simple, fast and specific one fluorometric method for determination of VLP in the presence of sofosbuvir was developed and validated. The fluorescence behavior of VLP in different organic solvents was examined and explained. Methanol was concluded to be the best sensitizing reagent. The native fluorescence intensity of VLP was accomplished at 383 nm with 339 nm for excitation wavelength. The impacts of experimental variables included pH, various organized media, and time of stability were examined and optimized. A linear relationship was achieved between the VLP concentration and the fluorescence intensity in a range of 5 to 5 × 103 ng mL-1 with 0.70 and 0.23 ng mL-1 , for quantitation and detection limits respectively. The proposed method was utilized for analyzing of VLP in human plasma and additionally expanded to examine the stability of VLP after its exposure to various stress conditions, like oxidative, alkaline, acidic, UV, daylight and sunlight conditions, according to ICH guidelines. Furthermore, the kinetics of acidic and oxidative degradations of VLP was examined...Continue Reading

References

Feb 3, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Li Xiao XuHuai You Wang
Jan 4, 2008·Pharmaceutical Research·Andrea HaweWim Jiskoot
Feb 7, 2017·Expert Opinion on Drug Metabolism & Toxicology·Teresa BrievaAntonio Rivero-Juarez
Sep 30, 2017·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Mahmoud A OmarMohamed A El Hamd

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Citations

Dec 19, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Z AydoğmuşG Yildiz
Dec 24, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Mohamed A Abdel-LateefSayed M Derayea
Dec 19, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Sayed M DerayeaEbtihal Samir
Feb 16, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ahmed H AbdelazimAhmed S Abu-Khadra
May 14, 2021·Critical Reviews in Analytical Chemistry·Ozge SelcukBengi Uslu
Jun 18, 2021·Critical Reviews in Analytical Chemistry·Sara I AborasMohamed A Korany

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