PMID: 26815344Jan 28, 2016Paper

Study on genotype and clinical characteristics of infection of carbapenemase-producing Enterobacter cloacae

Zhonghua yi xue za zhi
Qigang ZhaoYanhua Li

Abstract

To study the genotypes and clinical characteristics of carbapenemase-producing Enterobacter cloacae (E.cloacae), and lay the foundation for active control of nosocomial infection. E.cloacae isolates were collected from January 2007 to December 2014. Strains which showed decreased sensitivity to carbapenem were screened out by the modified Hodge test (MHT) and EDTA-disk synergy test. The genotype of blaKPC, blaIMP, blaVIM, blaOXA-48 and blaNDM-1 were detected by PCR amplication, the product of PCR was sequenced and conducted by Blast (http://blast.ncbi.nlm.nih.gov/Blast.cgi). Conjugal transfer experiment was conducted to prove horizontal transmit of carbapenemase gene produced by E.cloacae. Meanwhile, the clinical epidemiological data of patients infected by selected strains were also analyzed. Sixty-four carbapenemase producing E.cloacae were detected by MHT, EDTA-disk synergy test and PCR amplification. Forty-five strains (70.3%) out of 64 strains infection came from nosocomial infection, while 19 strains (29.7%) from the community infection. The strains were mainly isolated from secretions samples and sputum samples, which accounted for 65.6% (42/64) and 23.4% (15/64) separately. The mainly clinical departments were orthopaed...Continue Reading

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