PMID: 2488881Sep 1, 1989Paper

Study on high molecular weight androgen binding protein in submandibular gland of rat

The Bulletin of the Kanagawa Dental College : BKDC
M Tarui

Abstract

Testosterone (T) and 5 alpha-dihydrotestosterone (DHT) binding to cytosol and nuclear extract were investigated in the submandibular gland (SMG) and ventral prostate (VP) of rat at 3 and 7 weeks of age. The concentration of T and DHT were also measured by a specific radioimmunoassay (RIA) in the cytosol and nuclei of the tissues. The androgen binding protein analyzed by Sephacryl S-200 column chromatography showed a single binding peak in the nuclei and two critical peaks in the cytosol at 7 weeks old SMG and VP. The binding proteins were recovered predominantly as the mol wt of 150-200 kDa and 14-20 kDa in the cytosol and as a mol wt of 14-20 kDa in the nuclei. The two kinds of androgen binding protein were also revealed in the cytosol of 3 weeks old rat SMG. However, the S-200 column chromatography did not reveal the 150-200 kDa protein in the prostatic cytosol from 3 weeks rat, showing one binding peak of 14-20 kDa protein. The 150-200 kDa protein was specific to T in SMG at 3rd week and to DHT at 7th week in VP. The amount of specific binding of DHT to the 150-200 kDa protein was the greatest in VP cytosol of 7th week. The specific binding to the nuclear protein was shown by T in SMG at 3rd week and by DHT in VP at 7th week...Continue Reading

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