PMID: 11326954May 1, 2001Paper

Study on HSP70, 90 mRNA gene expression in peripheral blood mononuclear cells with steroid-resistant asthmatics

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases
YaobinY Zhu

Abstract

To investigate the role of heat shock protein(HSP) 70, 90 alpha, 90 beta mRNA on the pathogenesis of steroid-resistant(SR) asthma. 9 SR asthmadics 16 steroid sensitive (SS) asthmatics and 10 normal healthy volunteers were studied. With reverse transcription-polymerase chain reaction(RT-PCR), the expression of hsp70, 90 alpha, 90 beta mRNA were detected in peripheral blood mononuclear cell(PMBC) from normal volunteers and SR. steroid-sensitive(SS) asthmatics. Inhibition of PHA-induced lymphocyte proliferation by dexamethasones (Dex) was investigated using lymphocyte proliferation assay. There were no expression of hsp70mRNA in PMBC of normal volunteers; the levels of expression of hsp70, 90 alpha, 90 beta mRNA in PBMC of SR asthmatics (70 = 2.95 +/- 1.12, 90 alpha = 2.17 +/- 0.89, 90 beta = 2.22 +/- 0.83) were significantly higher than SS asthmatics (70 = 0.23 +/- 0.09, 90 alpha = 1.07 +/- 0.39, 90 beta = 0.94 +/- 0.32) (P < 0.01) and normal volunteers (70 = 0, 90 alpha = 0.45 +/- 0.19, 90 beta = 0.32 +/- 0.15) (P < 0.01); There were significantly positive correlation between the degree of inhibition by Dex(10(-7) mol/L) of SR, SS asthmatics of proliferation of T lymphocytes and the expression of hsp70, 90 alpha, 90 beta mRNA in...Continue Reading

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