Study on Human Urinary Metabolic Profiles after Consumption of Kale and Daikon Radish using a High-resolution Mass Spectrometry-Based Non-targeted and Targeted Metabolomic Approach

Journal of Agricultural and Food Chemistry
Jianghao SunPei Chen

Abstract

In the present study, urine samples were collected from healthy human volunteers to determine the metabolic fates of phenolic compounds and glucosinolates after a single meal of kale and daikon radish. The major glucosinolates and phenolic compounds in kale and daikon radish were measured. The urinary metabolome after feeding at different time periods was investigated. A targeted metabolite analysis method was developed based on the known metabolic pathways for glucosinolates and phenolic compounds. Using a targeted approach, a total of 18 metabolites were found in urine: 4 from phenolic compounds and 14 from glucosinolates. Among these metabolites, 4-methylsulfinyl-3-butenyl isothiocyanate, 4-methylsulfinyl-3-butenyl isothiocyanate-cysteine, and 4-methylsulfinyl-3-butenylglucosinolate-N-acetyl cysteine were reported for the first time in human urine. The combination of non-targeted and targeted metabolomic approaches can gain a full metabolite profile for human dietary intervention studies.

References

Feb 6, 2002·Nutrition·S Aisling Aherne, Nora M O'Brien
Jun 10, 2006·BMC Genomics·Robert A van den BergMariët J van der Werf
Feb 3, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Bethanne M WarrackDieter M Drexler
Feb 24, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Federico FerreresPaula B Andrade
May 9, 2009·International Journal of Food Sciences and Nutrition·Patrizia RisoMarisa Porrini
May 23, 2009·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Angélique StalmachAlan Crozier
Jul 25, 2009·Journal of Agricultural and Food Chemistry·Long-Ze Lin, James M Harnly
May 15, 2010·Journal of Agricultural and Food Chemistry·Long-Ze Lin, James M Harnly
Dec 24, 2010·The American Journal of Clinical Nutrition·Aifric O'SullivanLorraine Brennan
Jan 5, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·María Elena CarteaPablo Velasco
Aug 31, 2013·Nutrients·Surangi H Thilakarathna, H P Vasantha Rupasinghe
Jan 17, 2015·Journal of Agricultural and Food Chemistry·Jianghao SunPei Chen
Aug 14, 2015·The American Journal of Clinical Nutrition·William Mb EdmandsAugustin Scalbert
Oct 4, 2016·Journal of Agricultural and Food Chemistry·Yifei WangNicholi Vorsa
Jul 28, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ya-Qin WangHong-Ju He
Jan 27, 2018·Journal of Clinical Biochemistry and Nutrition·Akinori Yanaka
May 12, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhifeng LiChoon Nam Ong
Oct 15, 2018·The Journal of Nutritional Biochemistry·Jennifer L KaczmarekHannah D Holscher
Sep 1, 2019·Journal of the Science of Food and Agriculture·Vasile Coman, Dan C Vodnar
Sep 25, 2019·Metabolites·Ana K Rosen VollmarCaroline H Johnson

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