PMID: 2483525Jan 1, 1989Paper

[Study on karyotypes C-banding and Ag-NORs of Rana minimus and Hyla chinensis from Fuzhou].

Yi chuan xue bao = Acta genetica Sinica
J M Gao

Abstract

Using bone marrow preparing chromosome method, BSG technique and a quick Ag-stained NORs method, the conventional, C-banded and Ag-stained karyotypes of Rana minimus Ting et Ts'ai and Hyla chinensis Guenther were studied. The diploid number of R. minimus is 2n = 26, consisting of 5 pairs of large and 8 pairs of small chromosomes. A secondary constriction is observed at a position near the centromere on the short arm of chromosome No. 6 and it is also the position of the "standard NORs" Chromosome C-banding shows that the heterochromations of R. minimus are located in the centromeric and pericentromeric regions, with comparatively few lightly-stained interstitial C-bands and no telomeric ones. And the secondary constriction is C-banding positively stained. H. chinensis has 24 chromosomes which divide into a group of 6 large pairs and a group of 6 small pairs. A secondary constriction appears at a position on the long arm of chromosome No. 10. It is also the position of the "standard NORs" and C-banding positively stained. The C-banding pattern is mainly composed of centromeric C-bands with few interstitial ones and no telomeric ones. The karyotypic characters of Ranidae and Hylidae and the relationship to the evolution are also ...Continue Reading

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