Study on peptide hydrolysis by aminopeptidases from Streptomyces griseus, Streptomyces septatus and Aeromonas proteolytica

Applied Microbiology and Biotechnology
Jiro ArimaTadashi Hatanaka

Abstract

We developed a spectrophotometric assay for peptide hydrolysis by aminopeptidases (APs). The assay enables the measurement of free amino acids liberated by AP-catalyzed peptide hydrolysis using 4-aminoantipyrine, phenol, peroxidase, and L-amino acid oxidase. We investigated the specificity of bacterial APs [enzymes from Streptomyces griseus (SGAP), Streptomyces septatus (SSAP), and Aeromonas proteolytica (AAP)] toward peptide substrates using this assay method. Although these enzymes most efficiently cleave leucyl derivatives among 20 aminoacyl derivatives, in peptide hydrolysis, the catalytic efficiencies of Phe-Phe hydrolysis by SGAP and SSAP exceed that of Leu-Phe hydrolysis. Furthermore, all enzymes showed the maximum catalytic efficiencies for Phe-Phe-Phe hydrolysis. These results indicate that the hydrolytic activities of bacterial APs are affected by the nature of the penultimate residue or flanking moiety and the length of the peptide substrate.

References

Oct 1, 1975·Journal of Bacteriology·E P DesmondF J Behal
Jan 1, 1976·Methods in Enzymology·J M Prescott, S H Wilkes
Dec 1, 1988·Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie·G J SanderinkG Siest
Nov 15, 1981·Analytical Biochemistry·E DoiT Matoba
Oct 1, 1993·Molecular Biology Reports·W A Krajewski, S V Razin
Feb 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Taylor
Feb 7, 1997·Journal of Molecular Biology·H M GreenblattG Shoham
Apr 20, 2000·Acta Crystallographica. Section D, Biological Crystallography·R GilboaG Shoham
Dec 12, 2002·Chemical Reviews·W Todd Lowther, Brian W Matthews
Dec 31, 1954·Acta Physiologica Scandinavica·Y AVI-DORK G PAUL
May 13, 2004·The Journal of Biological Chemistry·Krzysztof P Bzymek, Richard C Holz
Sep 11, 2004·Protein Expression and Purification·Krzysztof P BzymekRichard C Holz
Sep 25, 2004·Acta Crystallographica. Section D, Biological Crystallography·Vera ReilandG Shoham

Citations

Feb 4, 2009·Applied Biochemistry and Biotechnology·Bo WuBin Yao
Jan 15, 2014·Enzyme and Microbial Technology·Arya Nandan, Kesavan Madhavan Nampoothiri
Jan 16, 2015·Open Biology·Veronica M JarockiSteven P Djordjevic
Nov 4, 2006·Applied Microbiology and Biotechnology·Tadashi HatanakaMasaki Iwabuchi
Nov 26, 2010·Current Microbiology·R RahulanKesavan Madhavan Nampoothiri
Sep 26, 2008·Protein Expression and Purification·Tadashi HatanakaMasaki Iwabuchi
Jun 18, 2009·The FEBS Journal·Jiro ArimaKenji Inagaki
Sep 25, 2007·Journal of Applied Microbiology·V NagyG Szakacs
Oct 31, 2012·Food Chemistry·Tadashi HatanakaTakafumi Mukaihara
Jul 29, 2008·In Vitro Cellular & Developmental Biology. Animal·Mohammad K KhalesiR H Wijffels
Oct 13, 2010·Applied Biochemistry and Biotechnology·Arya NandanKesavan Madhavan Nampoothiri
Jan 6, 2011·Applied Biochemistry and Biotechnology·Tadashi HatanakaYuya Kumagai
Apr 29, 2020·Applied Microbiology and Biotechnology·Arya Nandan, Kesavan Madhavan Nampoothiri
Jul 17, 2018·Applied Biochemistry and Biotechnology·Kun WanTadashi Hatanaka

Related Concepts

Aeromonas
Aminopeptidase
Hydrolysis
Polypeptides
Spectrophotometry
Streptomyces
Substrate Specificity

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