Apr 23, 1976

Study on proteins from yeast cytoplasmic ribosomes by two-dimensional gel electrophoresis

Molecular & General Genetics : MGG
J Ishiguro

Abstract

Proteins of yeast cytoplasmic ribosomes were analyzed by two different methods of two dimensional gel electrophoresis: run at pH 8.6 in 1-D1 and at pH 4.6 in 2-D (Method A); run at pH 5.0 in 1-D and in the presence of sodium dodecyl sulfate in 2-D (Method B). The numbers of proteins estimated were 28 (Method A) and 29 or 30 (Method B) in the 40S small subunit, and 40 (Method A) and 41 (Method B) in the 60S large subunit, respectively. Molecular weights of proteins in the small and the large subunits were found to be less than 40,000 and 60,000 respectively.

Mentioned in this Paper

Ribosomal Proteins
SDS-PAGE
Cytoplasmic
Ribosomes
Sodium Dodecyl Sulfate
Saccharomyces cerevisiae
Cytoplasm
Hydrogen-Ion Concentration
Electrophoresis, Gel, Two-Dimensional

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