Study on the inter-subspecies nuclear transfer of river buffalo somatic cell nuclei into swamp buffalo oocyte cytoplasm

Animal Reproduction Science
C Y YangX W Liang

Abstract

The objective of this study was to explore the feasibility of inter-subspecies somatic cell nuclear transfer (SCNT) of river buffalo (50 chromosomes) somatic cell nuclei into swamp buffalo (48 chromosomes) oocyte cytoplasm. The enucleated swamp buffalo oocytes were fused with four different types of river buffalo cells: freshly thawed ear fibroblasts, serum-starved ear fibroblasts, cumulus cells and ear fibroblasts from a cloned buffalo calf. As a result, the developmental competence of embryos reconstructed with freshly thawed ear fibroblasts was the poorest (P<0.01), while those of the other three types were not different from each other. Furthermore, the efficiency of swamp-swamp buffalo, swamp-river buffalo and bovine-buffalo SCNT were also compared. The results showed that the blastocyst rate of swamp-river reconstructed embryos was not different from swamp-swamp embryos, while significantly higher than that of bovine-buffalo embryos (P<0.01). A total of thirty cloned blastocysts derived from freshly thawed ear fibroblasts were transferred into thirteen recipient buffaloes, four recipients established pregnancy, while three of them aborted on Days 65, 75 and 90 of gestation, respectively. One cross-bred buffalo (Murrah x s...Continue Reading

References

Oct 3, 1999·Journal of Reproduction and Fertility·K D SinclairJ J Robinson
Jul 24, 2002·Biology of Reproduction·Da-Yuan ChenHe-Min Zhang
Dec 31, 2002·Molecular Reproduction and Development·H MenJ J Rutledge
Jan 22, 2004·Cloning and Stem Cells·Liangxue Lai, Randall S Prather
Jan 27, 2005·Cloning and Stem Cells·Martha C GómezBetsy L Dresser
Oct 14, 2006·Molecular Reproduction and Development·Chen Jian-QuanCheng Guo-Xiang
Apr 21, 2007·The Journal of Reproduction and Development·Ralf PoehlandSanjay Bhojwani
May 4, 2007·Biology of Reproduction·Deshun ShiQingyou Liu
May 21, 2008·The Journal of Reproduction and Development·Mayuko KuromeHiroshi Nagashima

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