Study on the interaction between hematoporphyrin monomethyl ether and DNA and the determination of hematoporphyrin monomethyl ether using the resonance light scattering technique

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Zhanguang ChenJunhui Chen

Abstract

The interaction between photosensitizer anticancer drug hematoporphyrin monomethyl ether (HMME) and ctDNA has been studied based on the decreased resonance light scattering (RLS) phenomenon. The RLS, UV-vis and fluorescence spectra characteristics of the HMME-ctDNA system were investigated. Besides, the phosphodiesters quaternary ammonium salt (PQAS), a kind of new gemini surfactant synthesized recently, was used to determine anticancer drug HMME based on the increasing RLS intensity. Under the optimum assay conditions, the enhanced RLS intensity was proportional to the concentration of HMME. The linear range was 0.8-8.4microgmL(-1), with correlation coefficient R(2)=0.9913. The detection limit was 0.014microgmL(-1). The human serum samples and urine samples were determined satisfactorily, which proved that this method was reliable and applicable in the determination of HMME in body fluid. The presented method was simple, sensitive and straightforward and could be a significant method in clinical analysis.

References

Feb 20, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Zhanguang ChenYali Han
Nov 18, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Changqun CaiHang Gong
Dec 9, 2008·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Zhanguang ChenZhiming Guo
Jan 15, 2010·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Changqun Cai, Xiaoming Chen

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Citations

Mar 20, 2013·Cancer Biotherapy & Radiopharmaceuticals·Xiaomin SuQuanhong Liu
Jul 26, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Haibo QiYuan Tian
Mar 31, 2015·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yu WangTingting Zhao
May 19, 2012·Photodiagnosis and Photodynamic Therapy·Yu PuZhengwei Yu

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