Study on the resonance light scattering spectrum of berberine-cetyltrimethylammonium bromide system and the determination of nucleic acids at nanogram levels

Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
Rutao LiuChangxia Sun

Abstract

The interaction of berberine with nucleic acid in the presence of cetyltrimethylammonium bromide (CTMAB) in aqueous solution has been studied by spectrophotometry and resonance light scattering (RLS) spectroscopy. At pH 7.30, the RLS signals of berberine were greatly enhanced by nucleic acid in the region of 300-600 nm characterized by four peaks at 324.0, 386.5, 416.5 and 465.0 nm. The binding properties were examined by using a Scatchard plot based on the measurement of enhanced RLS data at 416.5 nm. Under optimum conditions, the increase of RLS intensity of this system at 416.5 nm is proportional to the concentration of nucleic acid. The linear range is 7.5 x 10(-9)-7.5 x 10(-5) g ml(-1) for calf thymus DNA, 7.5 x 10(-9)-2.5 x 10(-5) g ml(-1) for herring sperm DNA, and 5.0 x 10(-9)-2.5 x 10(-5) g ml(-1) for yeast RNA. The detection limits (S/N = 3) are 2.1 ng ml(-1) for calf thymus DNA, 6.5 ng ml(-1) for herring sperm DNA and 3.5 ng ml(-1) for yeast RNA, respectively. Three synthetic samples were analyzed satisfactorily.

References

May 10, 1983·Biochemistry·R F PasternackJ J Villafranca
Dec 10, 1999·Biochemistry·J Ren, J B Chaires

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Citations

Dec 8, 2004·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xia WuYuxia Luan
Jun 14, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xia WuChangying Guo
Jun 14, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Aiyun LiDong Zheng
Apr 30, 2019·Luminescence : the Journal of Biological and Chemical Luminescence·Mingwei GuoZhiyi Yao
Jul 20, 2018·Analytical and Bioanalytical Chemistry·Aoli WenShu Chen
Jul 29, 2006·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Yanhua ChenHanqi Zhang

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