Study on the synthesis of complement components by human renal mesangial cells in culture. Assessment of the effect of cytokines.

Cytotechnology
V MontinaroF P Schena

Abstract

Renal mesangial cell (MC) cultures are easily established and widely used. MC produce some complement (C) regulatory proteins. We studied whether MC synthesize C components (C3, C5, C8). MC cultures were established from normal portions of cortices of nephrectomies for renal cancer. After growing to near-confluence in RPMI/17% FBS and resting for 24 h in RPMI/0.5% FBS, MC were stimulated up to 72 h with IL-1β or IL-6 (10, 100, 1000 U/ml). Neither C5 nor C8 were detected by ELISA. While C3 was present in supernatant under basal conditions (15.5-107.6 ng/10(6) cells/24h) in different MC lines. IL-1β up-regulated the synthesis by 2.4-4.5 folds, whereas IL-6 did not show any effect. C3 synthetic rate was 1,76 ng/h/10(6) cells under IL-1 stimulation versus basal rate of 0,37 ng/h/10(6) cells. MC production of C3, especially induced by IL-1 may have pathogenetic relevance in glomerulonephritis.

References

Aug 1, 1992·Kidney International·V MontinaroA Rifai
Apr 1, 1992·Journal of the American Society of Nephrology : JASN·F P SchenaV Montinaro
Jan 1, 1991·International Archives of Allergy and Applied Immunology·K RotherE W Rauterberg

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Citations

Dec 11, 2013·The Journal of Biological Chemistry·Shibnath GhatakSuniti Misra

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