Study on the synthesis of sucrose-6-acetate catalyzed by fructosyltransferase from Aspergillus oryzae

New Biotechnology
Yawei HanDuobin Mao

Abstract

The study had mainly investigated the synthesis of sucrose-6-acetate (s-6-a) in fructosyltransferase action. The synthesis reaction of s-6-a was performed between sucrose and glucose-6-acetate (g-6-a), and identified by high performance liquid chromatography (HPLC). According to the reaction of s-6-a catalyzed by fructosyltransferase from Aspergillus oryzae, the effect factors of reaction, such as the ratio of g-6-a to sucrose, temperature, time, pH, substrate and enzyme concentration in the reaction, were investigated. All results indicated that the fructosyltransferase could catalyze the s-6-a synthesis, and the optimal conditions of fructosyltransferase in reaction were 50°C, pH 6.2, 48h reaction time, 60% sucrose, 1:3 ratio of g-6-a to sucrose and 4.0mg/L concentration of enzyme. This study plays the important role in sucralose synthesis, because it is very cumbersome in the reported methods.

References

Oct 24, 2006·Journal of Biotechnology·Iraj GhaziFrancisco J Plou

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Citations

Jun 11, 2014·Critical Reviews in Food Science and Nutrition·Vandana BaliReeba Panesar
Jul 6, 2012·Natural Product Reports·David DaudéIsabelle André
Jul 15, 2020·Biotechnology Letters·Junqing QianYudong Shan

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