Study on the zona pellucida 4 (ZP4) gene sequence and its expression in the ovaries of patients with polycystic ovary syndrome

Journal of Endocrinological Investigation
B MeczekalskiA Gozdzicka-Jozefiak

Abstract

Polycystic ovary syndrome (PCOS) is a common endocrine disorder of unknown pathology, involving reproductive and metabolic abnormalities. Oocyte-specific genes are a group of genes expressed exclusively in ovarian tissue; therefore, they can play an important role in ovarian pathologies such as PCOS. The zona pellucida 4 (ZP4) gene encodes glycoprotein which is a part of the extracellular matrix of oocyte. We analyzed 87 patients with PCOS, which were divided into four groups depending on their phenotype. In each patient, we performed profound clinical and biochemical analysis, including the measurement of serum androgens. The ovarian tissue samples were used to perform a real-time polymerase chain reaction and immunohistochemical staining using anti-ZP4 monoclonal antibodies. The ZP4 gene was sequenced from peripheral lymphocytes. The expression of ZP4 was present in early antral follicles and was stronger in mature follicles. The subgroup of patients with eumenorrhea and without hyperandrogenism presented the highest expression of ZP4 in ovarian tissue. In one case, we found a mutation of the ZP4 gene. No correlations were found between the ZP4 expression level and biochemical or clinical indices. Data from this and animal st...Continue Reading

References

Jan 1, 1994·DNA Sequence : the Journal of DNA Sequencing and Mapping·J D HarrisA G Sacco
Dec 23, 2003·Human Reproduction·UNKNOWN Rotterdam ESHRE/ASRM-Sponsored PCOS consensus workshop group
May 15, 2004·Human Reproduction·L LefièvreC L R Barratt
Oct 2, 2009·Journal of Endocrinological Investigation·B Meczekalski
Sep 14, 2010·Reproductive Biology and Endocrinology : RB&E·Anasua GangulySatish K Gupta
Jan 29, 2013·Human Reproduction·F M PetitJ Auer
Mar 12, 2013·Fertility and Sterility·Daniel A Dumesic, Joanne S Richards
Mar 29, 2014·The New England Journal of Medicine·Hua-Lin HuangHong-Mei Xiao

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Citations

Dec 19, 2016·Trends in Endocrinology and Metabolism : TEM·Stefano PalombaGiovanni Battista La Sala
Jul 30, 2015·Scientific Reports·Shigang ZhaoZi-Jiang Chen
Mar 23, 2019·Investigative Ophthalmology & Visual Science·Min ChenKaijun Wang
Nov 21, 2018·Health Psychology Review·Sulamunn R M ColemanJoshua M Smyth
Aug 8, 2019·Death Studies·Kristine Berg TitlestadKari Dyregrov
Jun 27, 2017·Journal of Negative Results in Biomedicine·Taif A AzadKhaled K Abu-Amero
Feb 6, 2017·Journal of Negative Results in Biomedicine·Altaf A KondkarKhaled K Abu-Amero
Jan 20, 2021·Journal of Clinical Pathology·Xiaoli WeiZhongxian Lu
Mar 13, 2021·Biology of Reproduction·Ming-Hua ZengHong-Mei Xiao

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Methods Mentioned

BETA
ELISA
PCR

Software Mentioned

STATISTICA
BLAST
StatSoft

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