Studying DNA Double-Strand Break Repair: An Ever-Growing Toolbox

Frontiers in Molecular Biosciences
Alexandra C VítorSérgio F de Almeida

Abstract

To ward off against the catastrophic consequences of persistent DNA double-strand breaks (DSBs), eukaryotic cells have developed a set of complex signaling networks that detect these DNA lesions, orchestrate cell cycle checkpoints and ultimately lead to their repair. Collectively, these signaling networks comprise the DNA damage response (DDR). The current knowledge of the molecular determinants and mechanistic details of the DDR owes greatly to the continuous development of ground-breaking experimental tools that couple the controlled induction of DSBs at distinct genomic positions with assays and reporters to investigate DNA repair pathways, their impact on other DNA-templated processes and the specific contribution of the chromatin environment. In this review, we present these tools, discuss their pros and cons and illustrate their contribution to our current understanding of the DDR.

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Citations

Aug 29, 2020·Nucleic Acids Research·Lea Milling KorsholmDorthe Helena Larsen
Jun 13, 2020·Science·Darpan Medhi, Maria Jasin
Jul 8, 2020·Genes·Clara Forrer Charlier, Rodrigo A P Martins
Jan 14, 2021·The Biochemical Journal·Rashmi Panigrahi, J N Mark Glover
Mar 5, 2021·Molecular Cell·Roger S ZouTaekjip Ha
Sep 14, 2020·DNA Repair·Cathrine Broberg Vågbø, Geir Slupphaug
Apr 11, 2021·Nature Cell Biology·Aline Marnef, Gaëlle Legube
May 1, 2021·Journal of Clinical Medicine·Annika ReddigDirk Reinhold
Jul 23, 2021·Current Opinion in Genetics & Development·Tanya T Paull
Jul 30, 2021·Frontiers in Cell and Developmental Biology·Stephanie M AckersonJason A Stewart
Aug 23, 2021·DNA Repair·Chitra MohanJessica Tyler
Aug 28, 2021·Cells·Antonio Rivas-DomínguezManuel Luis Orta
Aug 22, 2021·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sophie RamadierAnnarita Miccio

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Methods Mentioned

BETA
Assay
proximity ligation assay
electrophoresis
immunoprecipitation
X-ray
Transgenic
nuclear translocation
ChIP
Break-Seq
ubiquitination

Software Mentioned

PFGE
iBLESS
BLISS
BLESS
RATaR
BrlTL

Related Concepts

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Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

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