Studying heating effects on desi ghee obtained from buffalo milk using fluorescence spectroscopy

PloS One
Naveed Ahmad, Muhammad Saleem

Abstract

Characterisation and thermal deterioration of desi ghee obtained from buffalo milk is presented for the first time using the potential of Fluorescence spectroscopy. The emission bands in non-heated desi ghee centred at 375 nm is labelled for vitamin D, 390 nm for vitamin K, 440-460 nm for isomers of conjugated linoleic acid (CLA), 490 nm for vitamin A and the region 620-700 nm is assigned to chlorophyll contents. Fluorescence emission spectra from all the heated and non-heated ghee samples were recorded using excitation wavelengths at 280, and 410 nm which were found best for getting maximum spectral signatures. Heating of desi ghee affects its molecular composition, however, the temperature range from 140 to 170°C may be defined safe for cooking /frying where it does not lose much of its molecular composition. Further, the rise in temperature induces prominent spectral variations which confirm the deterioration of valuable vitamins, isomers of CLA and chlorophyll contents. Fluorescence emission peak at 552 nm shows oxidation product and an increase in its intensity with the rise in temperature is observed. In order to classify heated samples at different temperatures, principal component analysis (PCA) has been applied on heat...Continue Reading

References

Jan 1, 1990·Annual Review of Nutrition·G W Burton, M G Traber
Nov 4, 2000·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·L D WhighamR L Atkinson
Feb 27, 2004·The American Journal of Clinical Nutrition·Michael F Holick
Feb 3, 2005·Journal of Agricultural and Food Chemistry·Maurizio ZandomeneghiChiara Caffarata
Mar 24, 2005·Chemistry and Physics of Lipids·Barbara MuikMaría José Ayora-Cañada
Jun 30, 2005·Analytical and Bioanalytical Chemistry·Rana CheikhousmanInés Birlouez-Aragon
Jul 21, 2005·Journal of Agricultural and Food Chemistry·Marc MeurensYvan Larondelle
Jun 15, 2007·Free Radical Biology & Medicine·Maret G Traber, Jeffrey Atkinson
Nov 21, 2007·Public Health Nutrition·Manuela-Belén SilveiraJavier Tébar
Jan 9, 2008·Circulation·Thomas J WangRamachandran S Vasan
Feb 1, 2008·The Proceedings of the Nutrition Society·Paul Amuna, Francis B Zotor
Dec 31, 2008·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Neil BinkleyJohn Suttie
Jul 31, 2009·The American Journal of Clinical Nutrition·Daniel ToméGilles Fromentin
Sep 3, 2009·Journal of Agricultural and Food Chemistry·Konstantina I PoulliConstantinos A Georgiou
Nov 19, 2009·Journal of Agricultural and Food Chemistry·Noelia TenaRamón Aparicio
Jun 24, 2010·Journal of Agricultural and Food Chemistry·Enrico ValliMauro Andrea Cremonini
Feb 3, 2012·Nutrition Research Reviews·Tracy A McCrorieM Barbara E Livingstone
May 8, 2015·Multiple Sclerosis : Clinical and Laboratory Research·Marianna CorteseKjell-Morten Myhr
Jul 1, 2017·Comprehensive Reviews in Food Science and Food Safety·Antonia Chiou, Nick Kalogeropoulos

❮ Previous
Next ❯

Methods Mentioned

BETA
Fluorescence
fluorescence spectroscopy
PCA

Software Mentioned

MatLab

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.