Studying inner ear protein-protein interactions using FRET and FLIM.

Brain Research
Richard HallworthJian Zuo

Abstract

Molecular genetic studies of the inner ear have recently revealed a large number of previously undescribed proteins, but their functions remain unclear. Optical methods such as FRET and FLIM are just beginning to be applied to the study of functional interactions between novel inner ear proteins. This review discusses the various methods for employing FRET and FLIM in protein-protein interaction studies, their advantages and pitfalls, with examples drawn from inner ear studies.

References

Jan 1, 1990·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·M C Holley, J F Ashmore
Jan 1, 1997·Methods in Enzymology·J Matko, M Edidin
Mar 24, 1999·Trends in Cell Biology·B A Pollok, R Heim
Mar 29, 2000·Biophysical Journal·P J VerveerP I Bastiaens
Aug 31, 2000·Analytical Biochemistry·G H PattersonB G Barisas
Feb 15, 2001·Nature Genetics·K P Steel, C J Kros
Sep 18, 2001·Methods : a Companion to Methods in Enzymology·R N DayF Schaufele
Oct 23, 2001·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S MurataJ R Lakowicz
Feb 12, 2002·Nature Reviews. Molecular Cell Biology·Peter Dallos, Bernd Fakler
Dec 4, 2002·Nature Reviews. Molecular Cell Biology·Jin ZhangRoger Y Tsien
Jun 28, 2003·FEBS Letters·Timo ZimmermannRainer Pepperkok
Jun 28, 2003·Biophysical Journal·Michael G EricksonDavid T Yue
Jul 25, 2003·Methods in Enzymology·R Heim
Oct 7, 2003·Annual Review of Genomics and Human Genetics·Thomas B Friedman, Andrew J Griffith
Nov 13, 2003·Biosensors & Bioelectronics·Darcy J LichlyterOrhan Soykan
Dec 17, 2003·Microscopy Research and Technique·Ye Chen, Ammasi Periasamy
Dec 23, 2003·Differentiation; Research in Biological Diversity·Ye ChenAmmasi Periasamy
Feb 24, 2004·Annual Review of Physiology·Peter G Gillespie, Janet L Cyr
Mar 3, 2004·Nature Biotechnology·Megan A RizzoDavid W Piston
May 26, 2004·Biochemical and Biophysical Research Communications·Takeharu Nagai, Atsushi Miyawaki
Jun 9, 2004·Biology of the Cell·Marion Peter, Simon M Ameer-Beg
Dec 14, 2004·Human Molecular Genetics·Avital AdatoChristine Petit
Feb 22, 2005·Current Opinion in Biotechnology·Horst Wallrabe, Ammasi Periasamy
Jul 7, 2005·The Journal of Cell Biology·Gregory J PazourGeorge B Witman
Jul 13, 2005·Current Opinion in Neurobiology·Peter G GillespieBechara Kachar
Aug 23, 2005·Biophysical Journal·Dhasakumar NavaratnamJoseph Santos-Sacchi
Dec 2, 2005·Biomaterials·Fabien PinaudShimon Weiss

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Citations

Jan 9, 2010·Cell Death and Differentiation·S MichiorriG Casari
Aug 3, 2010·Nature Methods·Vasilis Ntziachristos
May 15, 2008·Pediatric Critical Care Medicine : a Journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies·Jon KaufmanRobert H Friesen
May 27, 2009·Critical Reviews in Oncology/hematology·Gilles RousserieIgor Nabiev
Sep 10, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Raluca A Niesner, Anja E Hauser
Feb 28, 2009·Brain Research·Samson JamesdanielDonald Coling
Apr 20, 2007·Developmental Neurobiology·Xudong WuJian Zuo
Jul 5, 2011·Bioorganicheskaia khimiia·V A Oleĭnikov

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