Studying Smaller and Neglected Organisms in Modern Evolutionary Venomics Implementing RNASeq (Transcriptomics)-A Critical Guide

Toxins
Björn Marcus von Reumont

Abstract

Venoms are evolutionary key adaptations that species employ for defense, predation or competition. However, the processes and forces that drive the evolution of venoms and their toxin components remain in many aspects understudied. In particular, the venoms of many smaller, neglected (mostly invertebrate) organisms are not characterized in detail, especially with modern methods. For the majority of these taxa, even their biology is only vaguely known. Modern evolutionary venomics addresses the question of how venoms evolve by applying a plethora of -omics methods. These recently became so sensitive and enhanced that smaller, neglected organisms are now more easily accessible to comparatively study their venoms. More knowledge about these taxa is essential to better understand venom evolution in general. The methodological core pillars of integrative evolutionary venomics are genomics, transcriptomics and proteomics, which are complemented by functional morphology and the field of protein synthesis and activity tests. This manuscript focuses on transcriptomics (or RNASeq) as one toolbox to describe venom evolution in smaller, neglected taxa. It provides a hands-on guide that discusses a generalized RNASeq workflow, which can be ...Continue Reading

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Citations

Nov 7, 2019·Toxins·Carlos E Santibáñez-LópezLourival D Possani
Aug 9, 2020·Marine Drugs·Anna M L KlompenSérgio N Stampar
Aug 7, 2020·Biochemical Society Transactions·Andrew A Walker
Jun 28, 2019·Toxins·Esperanza Rivera-de-TorreSara García-Linares
Mar 7, 2021·Toxins·Stephanie Mouchbahani-Constance, Reza Sharif-Naeini
Jul 4, 2020·Biochemical Pharmacology·Helen C MendelMarkus Muttenthaler
Mar 23, 2019·Toxicon : Official Journal of the International Society on Toxinology·Juliane Monteiro Dos SantosCarla Lima

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Methods Mentioned

BETA
RNASeq
dissection

Software Mentioned

FastQC
BUSCO
Mascot
Segemehl
DEseq
gVolante
Corset
Trinity
NGen14
EdgeR

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