Studying the active site pocket of Staphylococcus hyicus lipase by site-directed mutagenesis

Biochemical and Biophysical Research Communications
R C ChangJei-Fu Shaw

Abstract

Site-directed mutagenesis of a previously constructed, recombinant Staphylococcus hyicus lipase (49 kDa) showed that Val363 played a role in catalysis and substrate-binding. In comparison with wild type enzyme, the 64% and 89% decrease in the catalytic efficiency (kcat/Km) of the V363N and V363A enzymes, respectively, were largely caused by a 3.5- and 5.5-fold increase in the substrate-binding affinity (Km), respectively. In comparison with wild type enzyme, a G371A enzyme showed a 40% decrease in the Km, suggesting that G371 was important for substrate-binding specificity. Site-directed mutagenesis of the active site Asp559 revealed that in comparison with wild type enzyme, a D559E enzyme exhibited a 47% decrease in the kcat/Km but a twofold increase in the Km for p-nitrophenyl butyrate, suggesting that Asp-559, a component of the catalytic triad, was involved in substrate-specificity.

Citations

Jan 11, 2001·Biochimica Et Biophysica Acta·A Svendsen

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