Studying the effect of single mismatches in primer and probe binding regions on amplification curves and quantification in real-time PCR

Journal of Microbiological Methods
Beate SüssIngeborg Hein

Abstract

Comparison of a broad range of characteristics of real-time PCR amplification curves yielded only slight alterations for low numbers of mismatches in the primer binding regions, resulting in a quantification error up to 63.12%. The effects were more pronounced for mismatches in the probe binding region and resulted in a quantification error up to 33%.

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Citations

Oct 23, 2010·Applied Microbiology and Biotechnology·Peter RossmanithMartin Wagner
Apr 17, 2013·Retrovirology·Alexander O PasternakBen Berkhout
Dec 10, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Kelly M HarkinsAnne C Stone
Oct 15, 2014·Journal of Virological Methods·M BaudL Bigarré
Jun 12, 2012·Biochemical and Biophysical Research Communications·Christopher B JacksonAndré Schaller
Sep 13, 2011·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Robert SöderlundAnna Aspán
Jan 17, 2014·Journal of Clinical Microbiology·Priscilla F GerberTanja Opriessnig
Jan 11, 2020·PloS One·Felipe-Andrés PiedraPedro A Piedra

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