Studying the folding process of the acylphosphatase from Sulfolobus solfataricus. A comparative analysis with other proteins from the same superfamily

Biochemistry
Francesco BemporadFabrizio Chiti

Abstract

The folding process of the acylphosphatase from Sulfolobus solfataricus (Sso AcP) has been followed, starting from the fully unfolded state, using a variety of spectroscopic probes, including intrinsic fluorescence, circular dichroism, and ANS binding. The results indicate that an ensemble of partially folded or misfolded species form rapidly on the submillisecond time scale after initiation of folding. This conformational ensemble produces a pronounced downward curvature in the Chevron plot, appears to possess a content of secondary structure similar to that of the native state, as revealed by far-UV circular dichroism, and appears to have surface-exposed hydrophobic clusters, as indicated by the ability of this ensemble to bind to 8-anilino-1-naphthalenesulfonic acid (ANS). Sso AcP folds from this conformational state with a rate constant of ca. 5 s(-1) at pH 5.5 and 37 degrees C. A minor slow exponential phase detected during folding (rate constant of 0.2 s(-1) under these conditions) is accelerated by cyclophilin A and is absent in a mutant of Sso AcP in which alanine replaces the proline residue at position 50. This indicates that for a lower fraction of Sso AcP molecules the folding process is rate-limited by the cis-tran...Continue Reading

References

Mar 20, 1992·Journal of Molecular Biology·A PastoreR J Williams
Jan 15, 1997·Structure·M M ThunnissenP Nordlund
Jan 1, 1996·Folding & Design·T Hubbard, A Tramontano
Feb 17, 2001·Current Opinion in Structural Biology·V GrantcharovaA L Horwich
Feb 17, 2001·Current Opinion in Structural Biology·K GunasekaranL M Gierasch
Feb 20, 2002·Journal of Molecular Biology·Stefan M LarsonKevin W Plaxco
Feb 20, 2002·Journal of Molecular Biology·Julie Hollien, Susan Marqusee
Jun 25, 2002·Journal of Molecular Biology·Raphael GueroisLuis Serrano
Dec 19, 2002·Journal of Molecular Biology·Ignacio E Sánchez, Thomas Kiefhaber
Feb 1, 2003·FEBS Letters·Donatella Degl'InnocentiGiampietro Ramponi

❮ Previous
Next ❯

Citations

Oct 6, 2006·Journal of Biomolecular NMR·Katiuscia PaganoAlessandra Corazza
Oct 4, 2008·Biochimica Et Biophysica Acta·Francesco BemporadFabrizio Chiti
Jun 21, 2012·Biophysical Journal·Francesco BemporadChristopher M Dobson
May 3, 2008·The EMBO Journal·Francesco BemporadFabrizio Chiti
Jun 22, 2017·Biophysical Journal·Francesco EliaFrancesco Bemporad
Jun 20, 2014·Biochemistry·Simona ContiFrancesco Bemporad

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.