Studying the genotoxicity of vincristine on human lymphocytes using comet assay, micronucleus assay and TCR gene mutation test in vitro

Toxicology
Wei JiangJiliang He

Abstract

The results of our previous investigation for workers occupationally exposed to vincristine (VCR) indicated that the genetic damage was detectable with comet assay, cytokinesis-block micronucleus (CBMN) assay and housekeeping gene mutation tests. In order to determine the results of above investigation and to inquire further the characteristics of genotoxicity of VCR, the cytogenetic effects of VCR on human lymphocytes were assessed with comet assay, CBMN assay and T-cell receptor (TCR) gene mutation test in vitro. The lymphocytes from two healthy donors were incubated for 24h at doses of 0.00, 0.01, 0.02, 0.04, and 0.08microgml(-1) VCR. The results of the present experiment showed that VCR not only could induce DNA damage, increase significantly micronucleus frequencies and the apoptotic cell ratios and decrease the nuclear division index (NDI) with dose-response relationship, but also could produce nucleoplasmic bridges (NPBs), a biomarker of DNA misrepair and/or telomere end-fusions and nuclear buds (NBUDs), a biomarker of elimination of amplified DNA and/or DNA repair complexes. Moreover, VCR could enhance TCR gene mutation frequency (Mf-TCR) of human lymphocytes. There was good correlation between the parameters (mean tail...Continue Reading

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Citations

Sep 30, 2014·International Journal of Environmental Research and Public Health·Thiago Aley Brites de FreitasSharbel Weidner Maluf
Apr 24, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Qingfeng ZhaiYuxin Zheng
Aug 4, 2015·Stem Cells International·Fabian LangMichael A Rieger
Aug 2, 2015·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Y ZerdoumiJ-M Flaman
Apr 24, 2012·Radiation Oncology·Clemens SeidelArndt-Christian Müller
Dec 11, 2013·Toxicology·Małgorzata M DobrzyńskaMarcin Kruszewski
Oct 28, 2016·Balkan Journal of Medical Genetics : BJMG·N M MhaidatK E Bani-Hani
Oct 22, 2019·Journal of Toxicology and Environmental Health. Part B, Critical Reviews·Nicholas BirkettDaniel Krewski

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