Studying the growth boundary and subsequent time to growth of pathogenic Escherichia coli serotypes by turbidity measurements

Food Microbiology
A ValeroG Zurera

Abstract

The presence of Escherichia coli in contaminated food products is commonly attributed to faecal contamination when they are improperly handled and/or when inactivation treatments fail. Adaptation of E. coli at low pH and a(w) levels can vary at different temperatures depending on the serotype, thus more detailed studies are needed. In this work, a screening to assess the growth of four pathogenic serotypes of E. coli (O55:H6; O59:H21; O158:H23 and O157:H7) was performed. Subsequently, boundary models were elaborated with the fastest serotype selected at different temperatures (8, 12 and 16 degrees C), and inoculum levels (2, 3 and 4log cfu/mL) as function of pH (7.00-5.00) and a(w) (0.999-0.960). Finally, the growth kinetics of E. coli was described in the conditions that allowed growth. Results obtained showed that the serotypes O157:H7 and O59:H21 did not grow at more stringent conditions (8 degrees C; pH 5.50), while the E. coli O158:H23 was the best adapted, resulting in faster growth. The logistic regression models presented a good adjustment to data observed since more than 96.7% of cases were correctly classified. The growth interface was shifted to more limited conditions as the inoculum size was higher. Detection times...Continue Reading

References

Sep 15, 1998·Journal of Theoretical Biology·J Baranyi
Nov 4, 1998·International Journal of Food Microbiology·K A KristT A McMeekin
Mar 23, 2000·International Journal of Food Microbiology·M C te Giffel, M H Zwietering
Apr 1, 2000·Applied and Environmental Microbiology·J C AugustinV Carlier
Jan 5, 2002·International Journal of Food Microbiology·T P RobinsonB M Mackey
Apr 28, 2005·International Journal of Food Microbiology·Yvan Le MarcJózsef Baranyi
Jun 4, 2005·Applied and Environmental Microbiology·L GuillierJ-C Augustin
Jul 12, 2005·International Journal of Food Microbiology·Konstantinos P Koutsoumanis, John N Sofos
Sep 5, 2006·Applied and Environmental Microbiology·A MétrisJ Baranyi
Oct 27, 2007·International Journal of Food Microbiology·P N SkandamisJ N Sofos
Jun 25, 2008·International Journal of Food Microbiology·Eva BidlasRonald J W Lambert
Sep 8, 2009·The Journal of Hospital Infection·Barbara M Lund, Sarah J O'Brien

❮ Previous
Next ❯

Citations

Dec 19, 2015·Food Microbiology·Dafni Dimakopoulou-PapazoglouKonstantinos P Koutsoumanis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.