PMID: 9448269Mar 14, 1998Paper

Studying the recruitment of Sp1 to the beta-globin promoter with an in vivo method: protein position identification with nuclease tail (PIN*POINT)

Proceedings of the National Academy of Sciences of the United States of America
J S LeeJ H Chung

Abstract

Transcription is thought to be regulated by recruitment of transcription factors, adaptors, and certain enzymes to cis-acting elements through protein-DNA interactions and protein-protein interactions. To better understand transcription, a method with the capability to detect in vivo recruitment of these individual proteins will be essential. Toward this end, we use a previously undescribed in vivo method that we term protein position identification with nuclease tail (PIN*POINT). In this method, a fusion protein composed of a chosen protein linked to a nonsequence-specific nuclease is expressed in vivo, and the binding of the protein to DNA is made detectable by the nuclease-induced cleavage near the binding site. In this article, we used the technique protein position identification with nuclease tail to study the effect of the beta-globin locus control region (LCR) and promoter elements on the recruitment of transcription factor Sp1 to the beta-globin promoter. We present evidence that the hypersensitive sites of the LCR synergistically enhance the recruitment of a multimeric Sp1 complex to the beta-globin promoter and that this may be accomplished by protein-protein interactions with proteins bound to the LCR, the upstream ...Continue Reading

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Citations

Oct 12, 2000·The Biochemical Journal·B HerzogD K Granner
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·J S LeeJ H Chung
Oct 28, 2005·Nucleic Acids Research·Sundar DuraiSrinivasan Chandrasegaran
Sep 16, 2004·Molecular and Cellular Biology·Siyuan SongJohn Kim Choi
Sep 24, 1999·Biological Chemistry·S Chandrasegaran, J Smith
Jun 17, 2003·Proceedings of the National Academy of Sciences of the United States of America·Christopher D CarvinMichael P Kladde
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·C H LeeJ H Chung
Oct 14, 2017·Briefings in Functional Genomics·Robert A Policastro, Gabriel E Zentner
Apr 9, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·C Simon ShelleyM Amin Arnaout
Oct 22, 2002·American Journal of Physiology. Cell Physiology·Shiuhyang KuoHarry S Nick
Sep 1, 1999·Proceedings of the National Academy of Sciences of the United States of America·M K KimJ H Chung
Jul 27, 2005·Biochemical and Biophysical Research Communications·Mala ManiSrinivasan Chandrasegaran

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