Studying the Role of AMPK in Autophagy.

Methods in Molecular Biology
Sarah KriegMarc Dohmen

Abstract

AMPK is an energy-sensing kinase and is required for the induction and progression of the autophagy process. In this chapter, we describe experimental approaches to study the steady state and flux of autophagy in response to AMPK activation. For this purpose, we provide detailed protocols for the measurement of general as well as AMPK-specific autophagy markers by immunoblot and immunofluorescence analysis.

Citations

Aug 1, 2019·Experimental and Therapeutic Medicine·Wentao ShiZhijian Zhang
Feb 27, 2020·Nature Communications·Marc DohmenJörg Vervoorts
Apr 1, 2020·Journal of Cellular and Molecular Medicine·Lin XiaolongZeng Zhaolin
May 20, 2021·Inflammation·Ming-Ye ChenDong-Yun Ouyang

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